In the course of the course of the SARS-CoV-2 pandemic studies of mutations with results on spreading and vaccine effectiveness emerged. Giant scale mutation evaluation utilizing fast SARS-CoV-2 Entire Genome Sequencing (WGS) is usually unavailable however may assist public well being organizations and hospitals in monitoring transmission and rising ranges of mutant strains.
Right here we report a novel WGS method for SARS-CoV-2, the EasySeq™ RC-PCR SARS-CoV-2 WGS equipment. By making use of a reverse complement polymerase chain response (RC-PCR), an Illumina library preparation is obtained in a single PCR, thereby saving time, assets and facilitating high-throughput screening.
Utilizing this WGS method, we evaluated SARS-CoV-2 range and doable transmission inside a bunch of 173 sufferers and healthcare employees (HCW) of the Radboud college medical heart throughout 2020. As a result of emergence of variants of concern, we screened SARS-CoV-2 optimistic samples in 2021 for identification of mutations and lineages.
With use of EasySeq™ RC-PCR SARS-CoV-2 WGS equipment we had been in a position to acquire dependable outcomes to substantiate outbreak clusters and moreover determine new beforehand unassociated hyperlinks in a significantly simpler workaround in comparison with present strategies.
Moreover, varied SARS-CoV-2 variants of curiosity had been detected amongst samples and validated in opposition to an Oxford Nanopore sequencing amplicon technique which illustrates this method is appropriate for surveillance and monitoring present circulating variants.
SARS-CoV-2 fast antigen take a look at compared to RT-PCR focusing on totally different genes: a real-life analysis amongst unselected sufferers in a regional hospital of Italy
We assessed the efficiency of Panbio Fast Antigen Detection (RAD) take a look at for the detection of SARS-CoV-2 an infection and we in contrast it with the routine RT-PCR-based molecular take a look at in a inhabitants of 4167 unselected sufferers admitted to IRCCS Sacro Cuore Don Calabria Hospital, Italy. Evaluation stratified by Ct worth of SARS-CoV-2 gene targets, indicated that antigen optimistic Ct values had been considerably decrease in comparison with antigen destructive values (p<0.0001).
Total, we discovered discordance in 140, examined destructive by RAD and optimistic by RT-PCR and in four resulted optimistic by RAD and destructive by RT-PCR. RAD take a look at achieved a sensitivity and specificity of 66.82% and 99.89% respectively.
PPV was proven to be 97.87% whereas the NPV was proven to be 97.62%. In our context, RAD take a look at confirmed a dependable diagnostic response in topics that displayed excessive Ct values, akin to excessive viral load, whereas low means was exhibited to determine optimistic case with medium-low Ct values, thus presenting low viral load and, the place confirmatory RT-PCR was wanted.
Our discovering helps the usage of RAD take a look at in actual life settings the place excessive quantity of swabs is being processed however with warning when deciphering optimistic take a look at lead to low prevalence setting. This text is protected by copyright. All rights reserved.
Novel real-time PCR primarily based assays for differentiating fall armyworm strains utilizing 4 single nucleotide polymorphisms
The autumn armyworm, Spodoptera frugiperda, is a polyphagous international pest with a choice for gramineous crops comparable to corn, sorghum and pasture grasses. This species is comprised of two morphologically similar however genetically distinct host strains often known as the corn and rice strains, which may complicate pest administration approaches.
Two molecular markers are generally used to distinguish between strains, nevertheless, discordance between these markers can result in inconclusive pressure identification. Right here, we used double digest restriction website related DNA sequencing to determine diagnostic single nucleotide polymorphisms (SNPs) with alleles distinctive to every pressure.
We then used these strain-specific SNPs to develop 4 real-time PCR primarily based TaqMan assays to quickly and reliably differentiate between strains and interstrain hybrids. These assays present a brand new software for differentiating between strains in field-collected samples, facilitating future research on pressure inhabitants dynamics and interstrain hybridization charges. Understanding the essential ecology of S. frugiperda strains is important to tell future administration methods.
Low-copy transgene detection utilizing nested digital PCR for gene doping management
Gene doping is prohibited for honest competitors in human and horse sports activities. One model of gene doping is the administration of an exogeneous gene, known as a transgene, to postnatal people and horses. Though many transgene detection strategies primarily based on quantitative PCR, together with real-time PCR and digital PCR, have been lately developed, it stays troublesome to reliably detect low-copy transgenes.
On this research, we developed and validated a nested digital PCR methodology to particularly detect low-copy transgenes. The nested digital PCR consists of 1) pre-amplification utilizing standard PCR and a couple of) droplet digital PCR detection utilizing a hydrolysis probe.
Utilizing 5, 10, 20, 60, and 120 transgene copies as template, 496.0, 1089.7, 1820.7, 4313.3, and 7840.Zero copies/μL respectively had been detected utilizing our nested digital PCR. Though excessive concentrations of phenol, proteinase Ok, ethanol, EDTA, heparin, and genomic DNA all inhibited pre-amplification, their results on the digital PCR detection had been restricted.
As soon as pre-amplification was profitable, even substitution of bases inside the primers and probes had minimal results on transgene detection. The nested digital PCR developed on this research efficiently detected low-copy transgenes and can be utilized to carry out a qualitative take a look at, indicating its usefulness within the prevention of false positives and false negatives in gene doping detection.
Novel Quadruplex PCR for detecting and genotyping cell colistin resistance genes in human samples
Since 2016, a number of cell colistin resistance (mcr) genes have been recognized worldwide. It is price noting that solely mcr-1, mcr-3, mcr-8, and mcr-10 have been reported remoted immediately from scientific samples which created larger threat to human well being than different mcr gene sorts.
A novel Quadruplex polymerase chain response (Quad-PCR) protocol was developed to detect and genotype transferable colistin-resistance genes (mcr-1, mcr-3, mcr-8, mcr-10) in Enterobacteria for scientific laboratory functions. The protocol was validated by testing 11 scientific isolates of Escherichia coli and three scientific isolates of Klebsiella of human origin, every effectively characterised and prospectively validated.
The Quad-PCR assay confirmed full concordance with whole-genome sequence knowledge and displayed increased sensitivity and 100% specificity. The Quad-PCR assay achieved genotyping of mcr alleles (as singleton and combination with double or triple gene sorts) described in a single take a look at.
Optimum allocation of PCR checks to minimise illness transmission via contact tracing and quarantine
PCR testing is a vital functionality for managing illness outbreaks, however it’s also a restricted useful resource and have to be used rigorously to make sure the data achieve from testing is efficacious. Testing has two broad makes use of for informing public well being coverage, particularly to trace epidemic dynamics and to cut back transmission by figuring out and managing circumstances.
On this work we develop a modelling framework to look at the consequences of take a look at allocation in an epidemic, with a concentrate on utilizing testing to minimise transmission. Utilizing the COVID-19 pandemic for example, we study how the variety of checks performed per day pertains to discount in illness transmission, within the context of logistical constraints on the testing system.
We present that if every day testing is above the routine capability of a testing system, which may trigger delays, then these delays can undermine efforts to cut back transmission via contact tracing and quarantine. This work highlights that the 2 targets of aiming to cut back transmission and aiming to determine all circumstances are totally different, and it’s doable that specializing in one could undermine attaining the opposite.
2 × Rapid Taq Master Mix |
P222-04 |
Vazyme |
50 ml (10×5ml) |
EUR 308.4 |
2 × Taq Plus Master Mix |
P211-01 |
Vazyme |
5 ml |
EUR 160.8 |
2 × Taq Plus Master Mix |
P211-02 |
Vazyme |
15 ml |
EUR 231.6 |
2 × Taq Plus Master Mix |
P211-03 |
Vazyme |
50 ml |
EUR 466.8 |
2 × Taq Master Mix (Dye Plus) |
P112-01 |
Vazyme |
5 ml |
EUR 140.4 |
2 × Taq Master Mix (Dye Plus) |
P112-02 |
Vazyme |
15 ml |
EUR 175.2 |
2 × Taq Master Mix (Dye Plus) |
P112-03 |
Vazyme |
50 ml |
EUR 283.2 |
2× EpiArt HS Taq Master Mix |
EM201-01 |
Vazyme |
1ml |
EUR 174 |
2× EpiArt HS Taq Master Mix |
EM201-02 |
Vazyme |
5 ml (5×1ml) |
EUR 363.6 |
2× EpiArt HS Taq Master Mix |
EM201-03 |
Vazyme |
15 ml (15×1ml) |
EUR 770.4 |
Taq PCR Master Mix (2X, Red Dye) |
BS9297 |
Bio Basic |
1ml |
EUR 101.76 |
|
Taq PCR Master Mix (2X, Red Dye) |
BS9298 |
Bio Basic |
5ml |
EUR 227.04 |
|
Taq PCR Master Mix (2X, Blue Dye) |
BS9295 |
Bio Basic |
1ml |
EUR 101.76 |
|
Taq PCR Master Mix (2X, Blue Dye) |
BS9296 |
Bio Basic |
5ml |
EUR 227.04 |
|
2×Taq PCR Master Mix(with dye) |
K1034-1 |
ApexBio |
1 ml |
EUR 87.6 |
2×Taq PCR Master Mix(with dye) |
K1034-100 |
ApexBio |
100×1 ml |
EUR 950.4 |
2×Taq PCR Master Mix(with dye) |
K1034-20 |
ApexBio |
20×1 ml |
EUR 338.4 |
2×Taq PCR Master Mix(with dye) |
K1034-5 |
ApexBio |
5×1 ml |
EUR 142.8 |
2×Taq PCR Master Mix(with dye) |
K1034-50 |
ApexBio |
50×1 ml |
EUR 616.8 |
2 × Taq Plus Master Mix (Dye Plus) |
P212-01 |
Vazyme |
5 ml |
EUR 159.6 |
2 × Taq Plus Master Mix (Dye Plus) |
P212-02 |
Vazyme |
15 ml |
EUR 231.6 |
2 × Taq Plus Master Mix (Dye Plus) |
P212-03 |
Vazyme |
50 ml |
EUR 466.8 |
3G Taq Master Mix for PAGE (Red Dye) |
P115-01 |
Vazyme |
5 ml |
EUR 30.93 |
3G Taq Master Mix for PAGE (Red Dye) |
P115-02 |
Vazyme |
10 × 5 ml |
EUR 232 |
2× EpiArt HS Taq Master Mix (Dye Plus) |
EM202-01 |
Vazyme |
1ml |
EUR 174 |
2× EpiArt HS Taq Master Mix (Dye Plus) |
EM202-02 |
Vazyme |
5 ml (5×1ml) |
EUR 363.6 |
2× EpiArt HS Taq Master Mix (Dye Plus) |
EM202-03 |
Vazyme |
15 ml (15×1ml) |
EUR 770.4 |
Taq Pro Universal SYBR qPCR Master Mix |
Q712-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 128.8 |
Taq Pro Universal SYBR qPCR Master Mix |
Q712-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 580 |
2 × Taq Plus Master Mix II (Dye Plus) |
P213-01 |
Vazyme |
5 ml |
EUR 160.8 |
2 × Taq Plus Master Mix II (Dye Plus) |
P213-02 |
Vazyme |
15 ml |
EUR 231.6 |
2 × Taq Plus Master Mix II (Dye Plus) |
P213-03 |
Vazyme |
50 ml |
EUR 466.8 |
Taqman Master Mix |
M1121-500 |
Biovision |
each |
EUR 529.2 |
Accuris Taq Master Mix Red Dye, 2X conc., 1000 reactions |
PR1001-R-1000 |
Benchmark Scientific |
1 PC |
EUR 364.9 |
Accuris Taq Master Mix Red Dye, 2X conc., 200 reactions |
PR1001-R-200 |
Benchmark Scientific |
1 PC |
EUR 88.3 |
Accuris Taq Plus Master Mix, 2X conc., 1000 reactions |
PR1001-TP-1000 |
Benchmark Scientific |
1 PC |
EUR 1182.9 |
Accuris Taq Plus Master Mix, 2X conc., 200 reactions |
PR1001-TP-200 |
Benchmark Scientific |
1 PC |
EUR 276.6 |
Accuris Hot Start Taq Master Mix, 2X conc., 1000 reactions |
PR1001-HS-1000 |
Benchmark Scientific |
1 PC |
EUR 659.1 |
Accuris Hot Start Taq Master Mix, 2X conc., 200 reactions |
PR1001-HS-200 |
Benchmark Scientific |
1 PC |
EUR 161.2 |
Taqman Master Mix-ROX |
M1124-500 |
Biovision |
each |
EUR 529.2 |
Accuris Taq Master Mix Red Dye, 2X conc., sample, 5 reactions |
PR1001-R-S |
Benchmark Scientific |
1 PC |
EUR 7.1 |
Accuris Taq Plus Master Mix, 2X conc., sample, 20 reactions |
PR1001-TP-S |
Benchmark Scientific |
1 PC |
EUR 7.1 |
Taqman Master Mix-iCycler |
M1122-500 |
Biovision |
each |
EUR 529.2 |
Taqman Master Mix-Multiplex |
M1125-500 |
Biovision |
each |
EUR 529.2 |
Accuris Hot Start Taq Master Mix Red Dye, 2X conc., 1000 reactions |
PR1001-HSR-1000 |
Benchmark Scientific |
1 PC |
EUR 659.1 |
Accuris Hot Start Taq Master Mix Red Dye, 2X conc., 200 reactions |
PR1001-HSR-200 |
Benchmark Scientific |
1 PC |
EUR 161.2 |
Taqman Master Mix-Low ROX |
M1123-500 |
Biovision |
each |
EUR 529.2 |
Accuris Hot Start Taq Master Mix Red Dye, 2X conc., sample, 5 reactions |
PR1001-HSR-S |
Benchmark Scientific |
1 PC |
EUR 7.1 |
Jade? Master Mix |
M1105-500 |
Biovision |
each |
EUR 529.2 |
2 × AceTaq Master Mix |
P411-01 |
Vazyme |
1 ml |
EUR 142.8 |
2 × AceTaq Master Mix |
P411-02 |
Vazyme |
5 ml |
EUR 189.6 |
2 × AceTaq Master Mix |
P411-03 |
Vazyme |
15 ml |
EUR 314.4 |
AceQ Probe Master Mix |
Q113-EN02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 113.86 |
AceQ Probe Master Mix |
Q113-EN03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 507.2 |
amfiSure PCR Master Mix |
P0311-010 |
GenDepot |
2x50 rxns |
EUR 151.2 |
amfiSure PCR Master Mix |
P0311-025 |
GenDepot |
5X50 rxns |
EUR 198 |
amfiSure PCR Master Mix |
P0311-050 |
GenDepot |
10X50 rxns |
EUR 262.8 |
amfiSure PCR Master Mix |
P0311-100 |
GenDepot |
10x1ml |
EUR 343.2 |
amfiSure PCR Master Mix |
P0311-125 |
GenDepot |
15X50 rxns |
EUR 333.6 |
amfiSure PCR Master Mix |
P0311-200 |
GenDepot |
20x1ml |
EUR 562.8 |
amfiSure PCR Master Mix |
P0311-250 |
GenDepot |
50x50 rxns |
EUR 902.4 |
amfiSure PCR Master Mix |
P0311-500 |
GenDepot |
100x50 rxns |
EUR 1509.6 |
amfiXpand PCR Master Mix |
P0331-010 |
GenDepot |
2X50 rxns |
EUR 181.2 |
amfiXpand PCR Master Mix |
P0331-025 |
GenDepot |
5X50 rxns |
EUR 327.6 |
amfiXpand PCR Master Mix |
P0331-050 |
GenDepot |
10X50 rxns |
EUR 537.6 |
amfiXpand PCR Master Mix |
P0331-250 |
GenDepot |
50x50 rxns |
EUR 2247.6 |
RT Master Mix for qPCR |
HY-K0510 |
MedChemExpress |
1 mL (100 rxns) |
EUR 349.2 |
2 × Phanta Max Master Mix |
P515-01 |
Vazyme |
1 ml |
EUR 174 |
2 × Phanta Max Master Mix |
P515-02 |
Vazyme |
5 ml |
EUR 322.8 |
2 × Phanta Max Master Mix |
P515-03 |
Vazyme |
15 ml |
EUR 690 |
AceQ U+ Probe Master Mix |
Q113-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 320.4 |
AceQ U+ Probe Master Mix |
Q113-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 1010.4 |
Fast Probe Master Mix (5000 rxn) |
31005-2 |
Biotium |
50x1ML |
EUR 4645.2 |
Description: Minimum order quantity: 1 unit of 50x1ML |
2 × Vazyme LAmp Master Mix |
P311-01 |
Vazyme |
1 ml |
EUR 164.4 |
2 × Vazyme LAmp Master Mix |
P311-02 |
Vazyme |
5 ml |
EUR 254.4 |
2 × Vazyme LAmp Master Mix |
P311-03 |
Vazyme |
15 ml |
EUR 481.2 |
SYBR Green qPCR Master Mix |
HY-K0501 |
MedChemExpress |
5 mL (500 rxns) |
EUR 315.6 |
AceQ qPCR Probe Master Mix |
Q112-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ qPCR Probe Master Mix |
Q112-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
2 × Phanta Flash Master Mix |
P510-01 |
Vazyme |
1 ml |
EUR 45.6 |
2 × Phanta Flash Master Mix |
P510-02 |
Vazyme |
5 × 1 ml |
EUR 124 |
2 × Phanta Flash Master Mix |
P510-03 |
Vazyme |
15 × 1 ml |
EUR 348 |
ChamQ SYBR qPCR Master Mix |
Q311-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 96.44 |
ChamQ SYBR qPCR Master Mix |
Q311-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 434.8 |
Fast Probe Master Mix (200 rxn) |
31005 |
Biotium |
2x1ML |
EUR 280.8 |
Description: Minimum order quantity: 1 unit of 2x1ML |
Fast Probe Master Mix (500 rxn) |
31005-1 |
Biotium |
5x1ML |
EUR 559.2 |
Description: Minimum order quantity: 1 unit of 5x1ML |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX* |
17282-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX* |
17283-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX* |
17284-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX* |
17285-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
amfiSure Prime PCR Master Mix |
P1311-025 |
GenDepot |
5X50 rxns |
EUR 193.2 |
amfiSure Prime PCR Master Mix |
P1311-050 |
GenDepot |
10X50 rxns |
EUR 262.8 |
amfiSure Prime PCR Master Mix |
P1311-125 |
GenDepot |
15X50 rxns |
EUR 327.6 |
amfiSure Prime PCR Master Mix |
P1311-250 |
GenDepot |
50x50 rxns |
EUR 886.8 |
amfiSure Prime PCR Master Mix |
P1311-500 |
GenDepot |
100x50 rxns |
EUR 1485.6 |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *High ROX* |
17286-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *High ROX* |
17287-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
Seamless cloning Master Mix (Kit) |
B632219 |
Bio Basic |
40RXN, 40prep |
EUR 319.96 |
|
amfiSure Advanced PCR Master Mix |
P2311-025 |
GenDepot |
5X50 rxns |
EUR 198 |
amfiSure Advanced PCR Master Mix |
P2311-050 |
GenDepot |
10X50 rxns |
EUR 267.6 |
amfiSure Advanced PCR Master Mix |
P2311-125 |
GenDepot |
15X50 rxns |
EUR 338.4 |
amfiSure Advanced PCR Master Mix |
P2311-250 |
GenDepot |
50x50 rxns |
EUR 902.4 |
amfiSure Advanced PCR Master Mix |
P2311-500 |
GenDepot |
100x50 rxns |
EUR 1534.8 |
TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
17282 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
17283 |
AAT Bioquest |
5 mL |
EUR 308 |
Animal Detection Probe Master Mix |
QV110-EN01 |
Vazyme |
400 rxns |
EUR 104 |
Animal Detection Probe Master Mix |
QV110-EN02 |
Vazyme |
800 rxns |
EUR 185.6 |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
17284 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
17285 |
AAT Bioquest |
5 mL |
EUR 308 |
PCR-EZ D-PCR MASTER MIX |
BS294 |
Bio Basic |
100RXN, 100prep |
EUR 112.2 |
|
TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
17286 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
17287 |
AAT Bioquest |
5 mL |
EUR 308 |
Gloria Nova HS 2x Master Mix |
RK20717 |
Abclonal |
1mL |
Ask for price |
|
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
17288-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
17289-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
amfiRivert cDNA Synthesis Master Mix |
R5101-050 |
GenDepot |
50 rxns |
EUR 498 |
amfiRivert cDNA Synthesis Master Mix |
R5101-100 |
GenDepot |
2X50 rxns |
EUR 1016.4 |
amfiRivert cDNA Synthesis Master Mix |
R5101-200 |
GenDepot |
4X50 rxns |
EUR 1453.2 |
2x SYBR Green qPCR Master Mix |
B21203 |
Bimake |
25 mL |
EUR 1027.2 |
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
2x SYBR Green qPCR Master Mix |
B21202 |
Bimake |
5 mL |
EUR 268.8 |
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency. |
2 × AceTaq Master Mix (Dye Plus) |
P412-01 |
Vazyme |
1 ml |
EUR 142.8 |
2 × AceTaq Master Mix (Dye Plus) |
P412-02 |
Vazyme |
5 ml |
EUR 189.6 |
2 × AceTaq Master Mix (Dye Plus) |
P412-03 |
Vazyme |
15 ml |
EUR 314.4 |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17290-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17291-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
amfiSure ONE PCR Master Mix(2X) |
P7000-005 |
GenDepot |
5x1 ml |
EUR 262.8 |
amfiSure ONE PCR Master Mix(2X) |
P7000-010 |
GenDepot |
10x1 ml |
EUR 380.4 |
amfiSure ONE PCR Master Mix(2X) |
P7000-050 |
GenDepot |
50x1 ml |
EUR 1453.2 |
amfiSure ONE PCR Master Mix(2X) |
P7000-100 |
GenDepot |
100x1 ml |
EUR 2522.4 |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17292-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17293-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17270-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
17271-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
Entrans 2X qPCR Probe Master Mix |
RK21208 |
Abclonal |
40 RXN |
Ask for price |
|
ChamQ SYBR Color qPCR Master Mix |
Q411-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 112.5 |
ChamQ SYBR Color qPCR Master Mix |
Q411-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 507.2 |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17272-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
17273-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
17288 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
17289 |
AAT Bioquest |
5 mL |
EUR 308 |
ChamQ Geno-SNP Probe Master Mix |
Q811-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 332.4 |
ChamQ Geno-SNP Probe Master Mix |
Q811-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 1107.6 |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17274-1mL |
AAT Bioquest |
1 mL |
EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
17275-5mL |
AAT Bioquest |
5 mL |
EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
AceQ Universal Probe Master Mix V2 |
Q513-EN02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 130.11 |
AceQ Universal Probe Master Mix V2 |
Q513-EN03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 579.6 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17290 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
17291 |
AAT Bioquest |
5 mL |
EUR 308 |
AceQ Universal SYBR qPCR Master Mix |
Q511-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
AceQ Universal SYBR qPCR Master Mix |
Q511-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17292 |
AAT Bioquest |
1 mL |
EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
17293 |
AAT Bioquest |
5 mL |
EUR 308 |
ChamQ Universal SYBR qPCR Master Mix |
Q711-02 |
Vazyme |
500 rxn (20 μl/rxn) |
EUR 265.2 |
ChamQ Universal SYBR qPCR Master Mix |
Q711-03 |
Vazyme |
2500 rxn (20 μl/rxn) |
EUR 775.2 |
amfiRivert II cDNA Synthesis Master Mix |
R5500-050 |
GenDepot |
50 rxns |
EUR 448.8 |
amfiRivert II cDNA Synthesis Master Mix |
R5500-100 |
GenDepot |
2x50rxns |
EUR 692.4 |
SYBR Green qPCR Master Mix (No ROX) |
HY-K0523 |
MedChemExpress |
5 mL (500 rxns ) |
EUR 308.4 |
2 × Phanta Max Master Mix (Dye Plus) |
P525-01 |
Vazyme |
1 ml |
EUR 174 |
2 × Phanta Max Master Mix (Dye Plus) |
P525-02 |
Vazyme |
5 ml |
EUR 322.8 |
To develop an efficient technique, the targets have to be clear and efficiency metrics should match the targets of the testing technique. If metrics don’t match the goals of the technique, then these metrics could incentivise actions that undermine attaining the goals.