SARS-CoV-2 whole-genome sequencing using reverse complement PCR: For easy, fast and accurate outbreak and variant analysis

In the course of the course of the SARS-CoV-2 pandemic studies of mutations with results on spreading and vaccine effectiveness emerged. Giant scale mutation evaluation utilizing fast SARS-CoV-2 Entire Genome Sequencing (WGS) is usually unavailable however may assist public well being organizations and hospitals in monitoring transmission and rising ranges of mutant strains.

Right here we report a novel WGS method for SARS-CoV-2, the EasySeq™ RC-PCR SARS-CoV-2 WGS equipment. By making use of a reverse complement polymerase chain response (RC-PCR), an Illumina library preparation is obtained in a single PCR, thereby saving time, assets and facilitating high-throughput screening.

Utilizing this WGS method, we evaluated SARS-CoV-2 range and doable transmission inside a bunch of 173 sufferers and healthcare employees (HCW) of the Radboud college medical heart throughout 2020. As a result of emergence of variants of concern, we screened SARS-CoV-2 optimistic samples in 2021 for identification of mutations and lineages.

With use of EasySeq™ RC-PCR SARS-CoV-2 WGS equipment we had been in a position to acquire dependable outcomes to substantiate outbreak clusters and moreover determine new beforehand unassociated hyperlinks in a significantly simpler workaround in comparison with present strategies.

Moreover, varied SARS-CoV-2 variants of curiosity had been detected amongst samples and validated in opposition to an Oxford Nanopore sequencing amplicon technique which illustrates this method is appropriate for surveillance and monitoring present circulating variants.

SARS-CoV-2 fast antigen take a look at compared to RT-PCR focusing on totally different genes: a real-life analysis amongst unselected sufferers in a regional hospital of Italy

We assessed the efficiency of Panbio Fast Antigen Detection (RAD) take a look at for the detection of SARS-CoV-2 an infection and we in contrast it with the routine RT-PCR-based molecular take a look at in a inhabitants of 4167 unselected sufferers admitted to IRCCS Sacro Cuore Don Calabria Hospital, Italy. Evaluation stratified by Ct worth of SARS-CoV-2 gene targets, indicated that antigen optimistic Ct values had been considerably decrease in comparison with antigen destructive values (p<0.0001).

Total, we discovered discordance in 140, examined destructive by RAD and optimistic by RT-PCR and in four resulted optimistic by RAD and destructive by RT-PCR. RAD take a look at achieved a sensitivity and specificity of 66.82% and 99.89% respectively.

PPV was proven to be 97.87% whereas the NPV was proven to be 97.62%. In our context, RAD take a look at confirmed a dependable diagnostic response in topics that displayed excessive Ct values, akin to excessive viral load, whereas low means was exhibited to determine optimistic case with medium-low Ct values, thus presenting low viral load and, the place confirmatory RT-PCR was wanted.

Our discovering helps the usage of RAD take a look at in actual life settings the place excessive quantity of swabs is being processed however with warning when deciphering optimistic take a look at lead to low prevalence setting. This text is protected by copyright. All rights reserved.

Novel real-time PCR primarily based assays for differentiating fall armyworm strains utilizing 4 single nucleotide polymorphisms

The autumn armyworm, Spodoptera frugiperda, is a polyphagous international pest with a choice for gramineous crops comparable to corn, sorghum and pasture grasses. This species is comprised of two morphologically similar however genetically distinct host strains often known as the corn and rice strains, which may complicate pest administration approaches.

Two molecular markers are generally used to distinguish between strains, nevertheless, discordance between these markers can result in inconclusive pressure identification. Right here, we used double digest restriction website related DNA sequencing to determine diagnostic single nucleotide polymorphisms (SNPs) with alleles distinctive to every pressure.

We then used these strain-specific SNPs to develop 4 real-time PCR primarily based TaqMan assays to quickly and reliably differentiate between strains and interstrain hybrids. These assays present a brand new software for differentiating between strains in field-collected samples, facilitating future research on pressure inhabitants dynamics and interstrain hybridization charges. Understanding the essential ecology of S. frugiperda strains is important to tell future administration methods.

Low-copy transgene detection utilizing nested digital PCR for gene doping management

Gene doping is prohibited for honest competitors in human and horse sports activities. One model of gene doping is the administration of an exogeneous gene, known as a transgene, to postnatal people and horses. Though many transgene detection strategies primarily based on quantitative PCR, together with real-time PCR and digital PCR, have been lately developed, it stays troublesome to reliably detect low-copy transgenes.

On this research, we developed and validated a nested digital PCR methodology to particularly detect low-copy transgenes. The nested digital PCR consists of 1) pre-amplification utilizing standard PCR and a couple of) droplet digital PCR detection utilizing a hydrolysis probe.

Utilizing 5, 10, 20, 60, and 120 transgene copies as template, 496.0, 1089.7, 1820.7, 4313.3, and 7840.Zero copies/μL respectively had been detected utilizing our nested digital PCR. Though excessive concentrations of phenol, proteinase Ok, ethanol, EDTA, heparin, and genomic DNA all inhibited pre-amplification, their results on the digital PCR detection had been restricted.

As soon as pre-amplification was profitable, even substitution of bases inside the primers and probes had minimal results on transgene detection. The nested digital PCR developed on this research efficiently detected low-copy transgenes and can be utilized to carry out a qualitative take a look at, indicating its usefulness within the prevention of false positives and false negatives in gene doping detection.

Novel Quadruplex PCR for detecting and genotyping cell colistin resistance genes in human samples

Since 2016, a number of cell colistin resistance (mcr) genes have been recognized worldwide. It is price noting that solely mcr-1, mcr-3, mcr-8, and mcr-10 have been reported remoted immediately from scientific samples which created larger threat to human well being than different mcr gene sorts.

A novel Quadruplex polymerase chain response (Quad-PCR) protocol was developed to detect and genotype transferable colistin-resistance genes (mcr-1, mcr-3, mcr-8, mcr-10) in Enterobacteria for scientific laboratory functions. The protocol was validated by testing 11 scientific isolates of Escherichia coli and three scientific isolates of Klebsiella of human origin, every effectively characterised and prospectively validated.

The Quad-PCR assay confirmed full concordance with whole-genome sequence knowledge and displayed increased sensitivity and 100% specificity. The Quad-PCR assay achieved genotyping of mcr alleles (as singleton and combination with double or triple gene sorts) described in a single take a look at.

Optimum allocation of PCR checks to minimise illness transmission via contact tracing and quarantine

PCR testing is a vital functionality for managing illness outbreaks, however it’s also a restricted useful resource and have to be used rigorously to make sure the data achieve from testing is efficacious. Testing has two broad makes use of for informing public well being coverage, particularly to trace epidemic dynamics and to cut back transmission by figuring out and managing circumstances.

On this work we develop a modelling framework to look at the consequences of take a look at allocation in an epidemic, with a concentrate on utilizing testing to minimise transmission. Utilizing the COVID-19 pandemic for example, we study how the variety of checks performed per day pertains to discount in illness transmission, within the context of logistical constraints on the testing system.

We present that if every day testing is above the routine capability of a testing system, which may trigger delays, then these delays can undermine efforts to cut back transmission via contact tracing and quarantine. This work highlights that the 2 targets of aiming to cut back transmission and aiming to determine all circumstances are totally different, and it’s doable that specializing in one could undermine attaining the opposite.

2 × Taq Plus Master Mix
P211-03	Vazyme	50 ml	EUR 466.8

2 × Rapid Taq Master Mix
P222-01	Vazyme	5 ml (5×1ml)	EUR 144

2 × Rapid Taq Master Mix
P222-02	Vazyme	15 ml (15×1ml)	EUR 183.6

2 × Rapid Taq Master Mix
P222-03	Vazyme	50 ml (50 x 1 ml)	EUR 308.4

2 × Rapid Taq Master Mix
P222-04	Vazyme	50 ml (10×5ml)	EUR 308.4

Accuris Taq Plus Master Mix
PR1001-TP-1000	Benchmark Scientific	1 PC	EUR 992.89

Accuris Taq Plus Master Mix
PR1001-TP-200	Benchmark Scientific	1 PC	EUR 291.41

Accuris Taq Plus Master Mix
PR1001-TP-S	Benchmark Scientific	1 PC	EUR 83.75

2× EpiArt HS Taq Master Mix
EM201-01	Vazyme	1ml	EUR 174

2× EpiArt HS Taq Master Mix
EM201-02	Vazyme	5 ml (5×1ml)	EUR 363.6

2× EpiArt HS Taq Master Mix
EM201-03	Vazyme	15 ml (15×1ml)	EUR 770.4

2 × Taq Master Mix (Dye Plus)
P112-01	Vazyme	5 ml	EUR 140.4

2 × Taq Master Mix (Dye Plus)
P112-02	Vazyme	15 ml	EUR 175.2

2 × Taq Master Mix (Dye Plus)
P112-03	Vazyme	50 ml	EUR 283.2

Accuris Hot Start Taq Master Mix
PR1001-HS-1000	Benchmark Scientific	1 PC	EUR 589.04

Accuris Hot Start Taq Master Mix
PR1001-HS-200	Benchmark Scientific	1 PC	EUR 203.36

Accuris Taq Master Mix Red Dye
PR1001-R-1000	Benchmark Scientific	1 PC	EUR 360.31

Accuris Taq Master Mix Red Dye
PR1001-R-200	Benchmark Scientific	1 PC	EUR 145.94

Accuris Taq Master Mix Red Dye
PR1001-R-S	Benchmark Scientific	1 PC	EUR 83.75

Taq PCR Master Mix (2X, Blue Dye)
BS9295	Bio Basic	1ml	EUR 101.76

Taq PCR Master Mix (2X, Blue Dye)
BS9296	Bio Basic	5ml	EUR 227.04

Taq PCR Master Mix (2X, Red Dye)
BS9297	Bio Basic	1ml	EUR 101.76

Taq PCR Master Mix (2X, Red Dye)
BS9298	Bio Basic	5ml	EUR 227.04

2 × Taq Plus Master Mix (Dye Plus)
P212-01	Vazyme	5 ml	EUR 159.6

2 × Taq Plus Master Mix (Dye Plus)
P212-02	Vazyme	15 ml	EUR 231.6

2 × Taq Plus Master Mix (Dye Plus)
P212-03	Vazyme	50 ml	EUR 466.8

2×Taq PCR Master Mix(with dye)
K1034-1	ApexBio	1 ml	EUR 87.6

2×Taq PCR Master Mix(with dye)
K1034-100	ApexBio	100×1 ml	EUR 950.4

2×Taq PCR Master Mix(with dye)
K1034-20	ApexBio	20×1 ml	EUR 338.4

2×Taq PCR Master Mix(with dye)
K1034-5	ApexBio	5×1 ml	EUR 142.8

2×Taq PCR Master Mix(with dye)
K1034-50	ApexBio	50×1 ml	EUR 616.8

2× EpiArt HS Taq Master Mix (Dye Plus)
EM202-01	Vazyme	1ml	EUR 174

2× EpiArt HS Taq Master Mix (Dye Plus)
EM202-02	Vazyme	5 ml (5×1ml)	EUR 363.6

2× EpiArt HS Taq Master Mix (Dye Plus)
EM202-03	Vazyme	15 ml (15×1ml)	EUR 770.4

2 × Taq Plus Master Mix II (Dye Plus)
P213-01	Vazyme	5 ml	EUR 160.8

2 × Taq Plus Master Mix II (Dye Plus)
P213-02	Vazyme	15 ml	EUR 231.6

2 × Taq Plus Master Mix II (Dye Plus)
P213-03	Vazyme	50 ml	EUR 466.8

Accuris Hot Start Taq Master Mix Red Dye
PR1001-HSR-1000	Benchmark Scientific	1 PC	EUR 589.04

Accuris Hot Start Taq Master Mix Red Dye
PR1001-HSR-200	Benchmark Scientific	1 PC	EUR 203.36

Accuris Hot Start Taq Master Mix Red Dye
PR1001-HSR-S	Benchmark Scientific	1 PC	EUR 83.75

Green Taq Mix
P131-01	Vazyme	5 ml	EUR 142.8

Green Taq Mix
P131-02	Vazyme	15 ml	EUR 180

Green Taq Mix
P131-03	Vazyme	50 ml	EUR 297.6

Jade? Master Mix
M1105-500	Biovision	each	EUR 529.2

Taqman Master Mix
M1121-500	Biovision	each	EUR 529.2

amfiSure PCR Master Mix
P0311-010	GenDepot	2x50 rxns	EUR 151.2

amfiSure PCR Master Mix
P0311-025	GenDepot	5X50 rxns	EUR 198

amfiSure PCR Master Mix
P0311-050	GenDepot	10X50 rxns	EUR 262.8

amfiSure PCR Master Mix
P0311-100	GenDepot	10x1ml	EUR 343.2

amfiSure PCR Master Mix
P0311-125	GenDepot	15X50 rxns	EUR 333.6

amfiSure PCR Master Mix
P0311-200	GenDepot	20x1ml	EUR 562.8

amfiSure PCR Master Mix
P0311-250	GenDepot	50x50 rxns	EUR 902.4

amfiSure PCR Master Mix
P0311-500	GenDepot	100x50 rxns	EUR 1509.6

amfiXpand PCR Master Mix
P0331-010	GenDepot	2X50 rxns	EUR 181.2

amfiXpand PCR Master Mix
P0331-025	GenDepot	5X50 rxns	EUR 327.6

amfiXpand PCR Master Mix
P0331-050	GenDepot	10X50 rxns	EUR 537.6

amfiXpand PCR Master Mix
P0331-250	GenDepot	50x50 rxns	EUR 2247.6

2 × AceTaq Master Mix
P411-01	Vazyme	1 ml	EUR 142.8

2 × AceTaq Master Mix
P411-02	Vazyme	5 ml	EUR 189.6

2 × AceTaq Master Mix
P411-03	Vazyme	15 ml	EUR 314.4

Taqman Master Mix-iCycler
M1122-500	Biovision	each	EUR 529.2

Taqman Master Mix-ROX
M1124-500	Biovision	each	EUR 529.2

Taqman Master Mix-Multiplex
M1125-500	Biovision	each	EUR 529.2

Volatile Organics Mix
60-BIG-MIX	Scientific Laboratory Supplies	1ML	EUR 120.84

5 Component Mix
AOAC-MIX-1	Scientific Laboratory Supplies	EACH	EUR 117.42

Seamless cloning Master Mix (Kit)
B632219	Bio Basic	40RXN, 40prep	EUR 319.96

amfiSure Prime PCR Master Mix
P1311-025	GenDepot	5X50 rxns	EUR 193.2

amfiSure Prime PCR Master Mix
P1311-050	GenDepot	10X50 rxns	EUR 262.8

amfiSure Prime PCR Master Mix
P1311-125	GenDepot	15X50 rxns	EUR 327.6

amfiSure Prime PCR Master Mix
P1311-500	GenDepot	100x50 rxns	EUR 1485.6

amfiSure Advanced PCR Master Mix
P2311-025	GenDepot	5X50 rxns	EUR 198

amfiSure Advanced PCR Master Mix
P2311-050	GenDepot	10X50 rxns	EUR 267.6

amfiSure Advanced PCR Master Mix
P2311-125	GenDepot	15X50 rxns	EUR 338.4

amfiSure Advanced PCR Master Mix
P2311-250	GenDepot	50x50 rxns	EUR 902.4

amfiSure Advanced PCR Master Mix
P2311-500	GenDepot	100x50 rxns	EUR 1534.8

2 × Vazyme LAmp Master Mix
P311-01	Vazyme	1 ml	EUR 164.4

2 × Vazyme LAmp Master Mix
P311-02	Vazyme	5 ml	EUR 254.4

2 × Vazyme LAmp Master Mix
P311-03	Vazyme	15 ml	EUR 481.2

2 × Phanta Max Master Mix
P515-01	Vazyme	1 ml	EUR 174

2 × Phanta Max Master Mix
P515-02	Vazyme	5 ml	EUR 322.8

2 × Phanta Max Master Mix
P515-03	Vazyme	15 ml	EUR 690

SYBR Green qPCR Master Mix
HY-K0501	MedChemExpress	5 mL (500 rxns)	EUR 315.6

RT Master Mix for qPCR
HY-K0510	MedChemExpress	1 mL (100 rxns)	EUR 349.2

AceQ qPCR Probe Master Mix
Q112-02	Vazyme	500 rxn (20 μl/rxn)	EUR 265.2

AceQ qPCR Probe Master Mix
Q112-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 775.2

AceQ U+ Probe Master Mix
Q113-02	Vazyme	500 rxn (20 μl/rxn)	EUR 320.4

AceQ U+ Probe Master Mix
Q113-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 1010.4

amfiRivert cDNA Synthesis Master Mix
R5101-050	GenDepot	50 rxns	EUR 498

amfiRivert cDNA Synthesis Master Mix
R5101-100	GenDepot	2X50 rxns	EUR 1016.4

amfiRivert cDNA Synthesis Master Mix
R5101-200	GenDepot	4X50 rxns	EUR 1453.2

Taqman Master Mix-Low ROX
M1123-500	Biovision	each	EUR 529.2

Semi-volatile Organics Mix
76-BIG-MIX	Scientific Laboratory Supplies	1ML	EUR 150.48

Taq 5x PCR Mix 12.5 mM
BT10402	Bioatlas	250rxn	EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq 2x PCR Mix 100 rxn
BA01501	Bioatlas	100rxn	EUR 112.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Volatile Organics Mix (76 Components)
8260-BIG-MIX	Scientific Laboratory Supplies	1ML	EUR 296.4

PCR-EZ D-PCR MASTER MIX
BS294	Bio Basic	100RXN, 100prep	EUR 112.2

Fast Probe Master Mix (200 rxn)
31005	Biotium	2x1ML	EUR 280.8
Description: Minimum order quantity: 1 unit of 2x1ML

Fast Probe Master Mix (500 rxn)
31005-1	Biotium	5x1ML	EUR 559.2
Description: Minimum order quantity: 1 unit of 5x1ML

Fast Probe Master Mix (5000 rxn)
31005-2	Biotium	50x1ML	EUR 4645.2
Description: Minimum order quantity: 1 unit of 50x1ML

Pfu 2x Master Mix - 200 Reactions
3326	Intact Genomics	1/EA	EUR 360

2x SYBR Green qPCR Master Mix
B21202	Bimake	5 mL	EUR 268.8
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency.

2x SYBR Green qPCR Master Mix
B21203	Bimake	25 mL	EUR 1027.2
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency.

2 × AceTaq Master Mix (Dye Plus)
P412-01	Vazyme	1 ml	EUR 142.8

2 × AceTaq Master Mix (Dye Plus)
P412-02	Vazyme	5 ml	EUR 189.6

2 × AceTaq Master Mix (Dye Plus)
P412-03	Vazyme	15 ml	EUR 314.4

amfiSure ONE PCR Master Mix(2X)
P7000-005	GenDepot	5x1 ml	EUR 262.8

amfiSure ONE PCR Master Mix(2X)
P7000-010	GenDepot	10x1 ml	EUR 380.4

amfiSure ONE PCR Master Mix(2X)
P7000-050	GenDepot	50x1 ml	EUR 1453.2

amfiSure ONE PCR Master Mix(2X)
P7000-100	GenDepot	100x1 ml	EUR 2522.4

AceQ Universal SYBR qPCR Master Mix
Q511-02	Vazyme	500 rxn (20 μl/rxn)	EUR 265.2

AceQ Universal SYBR qPCR Master Mix
Q511-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 775.2

ChamQ Universal SYBR qPCR Master Mix
Q711-02	Vazyme	500 rxn (20 μl/rxn)	EUR 265.2

ChamQ Universal SYBR qPCR Master Mix
Q711-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 775.2

ChamQ Geno-SNP Probe Master Mix
Q811-02	Vazyme	500 rxn (20 μl/rxn)	EUR 332.4

ChamQ Geno-SNP Probe Master Mix
Q811-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 1107.6

amfiRivert II cDNA Synthesis Master Mix
R5500-050	GenDepot	50 rxns	EUR 448.8

amfiRivert II cDNA Synthesis Master Mix
R5500-100	GenDepot	2x50rxns	EUR 692.4

amfiRivert cDNA Synthesis Prime Master Mix
R5610-050	GenDepot	50 rxns	EUR 494.4

amfiRivert cDNA Synthesis Prime Master Mix
R5610-100	GenDepot	2x50 rxns	EUR 805.2

amfiRivert cDNA Synthesis Prime Master Mix
R5610-200	GenDepot	4X50 rxns	EUR 1453.2

amfiRivert cDNA Synthesis Prime Master Mix
R5610-500	GenDepot	10x50 rxns	EUR 3057.6

Maximo Dry-Master Mix (Lyophilized Polymerase)
S295	GeneOn	192 rcs (20µl, in plates or tubes)	EUR 159.6

Gloria Nova HS 2x Master Mix
RK20717	Abclonal	1mL	Ask for price

Entrans 2X qPCR Probe Master Mix
RK21208	Abclonal	40 RXN	Ask for price

Taq DNA Polymerase with dNTP Mix (6000U)
9K-001-0018	Bio Basic	6000U	EUR 1536.74

Taq DNA Polymerase with dNTP Mix (500U)
9K-001-0031	Bio Basic	500U	EUR 351.28

Taq DNA Polymerase with dNTP Mix (3000U)
9K-001-0032	Bio Basic	3000U	EUR 1088.87

Taq DNA Polymerase with dNTP Mix (1000U)
9K-001-0034	Bio Basic	1000U	EUR 518.84

HotStart PCR Master mix (2X, Green Dye)
BS9291	Bio Basic	5X1mL, 5ml	EUR 251.4

HotStart PCR Master mix (2X, Green Dye)
BS9292	Bio Basic	1ml	EUR 115.68

Reverse Transcriptase RT PCR Master Mix (5X)
MB1002-100reactions	BosterBio	100 reactions	EUR 616.8

Reverse Transcriptase RT PCR Master Mix (5X)
MB1002-200reactions	BosterBio	200 reactions	EUR 895.2

Reverse Transcriptase RT PCR Master Mix (5X)
MB1002-25reactions	BosterBio	25 reactions	EUR 226.8

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-010	GenDepot	2x50 rxns	EUR 303.6

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-025	GenDepot	5X50 rxns	EUR 546

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-050	GenDepot	10X50 rxns	EUR 934.8

amfiFusion High Fidelity PCR Master Mix(2X)
P0342-100	GenDepot	20x50 rxns	EUR 1599.6

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-001	GenDepot	1ml	EUR 336

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-002	GenDepot	2x1ml	EUR 578.4

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-005	GenDepot	5x1ml	EUR 993.6

amfiSure Ultra Fidelity PCR Master Mix(2X)
P0346-010	GenDepot	10x1ml	EUR 1632

2 × Vazyme LAmp Master Mix (Dye Plus)
P312-01	Vazyme	1 ml	EUR 165.6

2 × Vazyme LAmp Master Mix (Dye Plus)
P312-02	Vazyme	5 ml	EUR 254.4

2 × Vazyme LAmp Master Mix (Dye Plus)
P312-03	Vazyme	15 ml	EUR 481.2

2 × Phanta Max Master Mix (Dye Plus)
P525-01	Vazyme	1 ml	EUR 174

2 × Phanta Max Master Mix (Dye Plus)
P525-02	Vazyme	5 ml	EUR 322.8

2 × Phanta Max Master Mix (Dye Plus)
P525-03	Vazyme	15 ml	EUR 690

miRNA Universal SYBR® qPCR Master Mix
MQ101-01	Vazyme	125 rxn(20 μl/rxn)	EUR 165.6

miRNA Universal SYBR® qPCR Master Mix
MQ101-02	Vazyme	500 rxn(20 μl/rxn)	EUR 290.4

SYBR Green qPCR Master Mix (High ROX)
HY-K0521	MedChemExpress	1 mL (100 rxns)	EUR 135.6

SYBR Green qPCR Master Mix (Low ROX)
HY-K0522	MedChemExpress	5 mL (500 rxns )	EUR 308.4

SYBR Green qPCR Master Mix (No ROX)
HY-K0523	MedChemExpress	5 mL (500 rxns )	EUR 308.4

AceQ qPCR SYBR® Green Master Mix
Q111-02	Vazyme	500 rxn (20 μl/rxn)	EUR 265.2

AceQ qPCR SYBR® Green Master Mix
Q111-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 775.2

AceQ U+ Universal Probe Master Mix V2
Q513-02	Vazyme	500 rxn (20 μl/rxn)	EUR 320.4

AceQ U+ Universal Probe Master Mix V2
Q513-03	Vazyme	2500 rxn (20 μl/rxn)	EUR 1010.4

amfiRivert Sensi cDNA Synthesis Master Mix(4X)
R6200-050	GenDepot	50 rxns	EUR 400.8

amfiRivert Sensi cDNA Synthesis Master Mix(4X)
R6200-100	GenDepot	2X50 rxns	EUR 643.2

amfiRivert Sensi cDNA Synthesis Master Mix(4X)
R6200-200	GenDepot	4x50 rxns	EUR 1048.8

amfiRivert Sensi cDNA Synthesis Master Mix(4X)
R6200-500	GenDepot	10X50 rxns	EUR 1842

illustra Hot Start Master Mix 100 reactions
25150001	Scientific Laboratory Supplies	EACH	EUR 135.66

Accuris High Fidelity Master Mix, 200 rxns
PR1001-HF-200	Benchmark Scientific	1 each	EUR 175.4

Accuris High Fidelity Master Mix, 500 rxns
PR1001-HF-500	Benchmark Scientific	1 each	EUR 389.6

Volatile Organics Mix (Low Level)
60-BIG-MIX-200	Scientific Laboratory Supplies	1ML	EUR 116.28

Volatile Organics Mix (High Level)
60-BIG-MIX-2000	Scientific Laboratory Supplies	1ML	EUR 165.3

Fast EvaGreen Master Mix for qPCR(200 rxn)
31003	Biotium	2x1ML	EUR 244.8
Description: Minimum order quantity: 1 unit of 2x1ML

To develop an efficient technique, the targets have to be clear and efficiency metrics should match the targets of the testing technique. If metrics don’t match the goals of the technique, then these metrics could incentivise actions that undermine attaining the goals.