SARS-CoV-2 whole-genome sequencing using reverse complement PCR: For easy, fast and accurate outbreak and variant analysis

SARS-CoV-2 whole-genome sequencing using reverse complement PCR: For easy, fast and accurate outbreak and variant analysis
In the course of the course of the SARS-CoV-2 pandemic studies of mutations with results on spreading and vaccine effectiveness emerged. Giant scale mutation evaluation utilizing fast SARS-CoV-2 Entire Genome Sequencing (WGS) is usually unavailable however may assist public well being organizations and hospitals in monitoring transmission and rising ranges of mutant strains.
Right here we report a novel WGS method for SARS-CoV-2, the EasySeq™ RC-PCR SARS-CoV-2 WGS equipment. By making use of a reverse complement polymerase chain response (RC-PCR), an Illumina library preparation is obtained in a single PCR, thereby saving time, assets and facilitating high-throughput screening.
Utilizing this WGS method, we evaluated SARS-CoV-2 range and doable transmission inside a bunch of 173 sufferers and healthcare employees (HCW) of the Radboud college medical heart throughout 2020. As a result of emergence of variants of concern, we screened SARS-CoV-2 optimistic samples in 2021 for identification of mutations and lineages.
With use of EasySeq™ RC-PCR SARS-CoV-2 WGS equipment we had been in a position to acquire dependable outcomes to substantiate outbreak clusters and moreover determine new beforehand unassociated hyperlinks in a significantly simpler workaround in comparison with present strategies.
Moreover, varied SARS-CoV-2 variants of curiosity had been detected amongst samples and validated in opposition to an Oxford Nanopore sequencing amplicon technique which illustrates this method is appropriate for surveillance and monitoring present circulating variants.

SARS-CoV-2 fast antigen take a look at compared to RT-PCR focusing on totally different genes: a real-life analysis amongst unselected sufferers in a regional hospital of Italy

We assessed the efficiency of Panbio Fast Antigen Detection (RAD) take a look at for the detection of SARS-CoV-2 an infection and we in contrast it with the routine RT-PCR-based molecular take a look at in a inhabitants of 4167 unselected sufferers admitted to IRCCS Sacro Cuore Don Calabria Hospital, Italy. Evaluation stratified by Ct worth of SARS-CoV-2 gene targets, indicated that antigen optimistic Ct values had been considerably decrease in comparison with antigen destructive values (p<0.0001).
Total, we discovered discordance in 140, examined destructive by RAD and optimistic by RT-PCR and in four resulted optimistic by RAD and destructive by RT-PCR. RAD take a look at achieved a sensitivity and specificity of 66.82% and 99.89% respectively.
PPV was proven to be 97.87% whereas the NPV was proven to be 97.62%. In our context, RAD take a look at confirmed a dependable diagnostic response in topics that displayed excessive Ct values, akin to excessive viral load, whereas low means was exhibited to determine optimistic case with medium-low Ct values, thus presenting low viral load and, the place confirmatory RT-PCR was wanted.
Our discovering helps the usage of RAD take a look at in actual life settings the place excessive quantity of swabs is being processed however with warning when deciphering optimistic take a look at lead to low prevalence setting. This text is protected by copyright. All rights reserved.

Novel real-time PCR primarily based assays for differentiating fall armyworm strains utilizing 4 single nucleotide polymorphisms

The autumn armyworm, Spodoptera frugiperda, is a polyphagous international pest with a choice for gramineous crops comparable to corn, sorghum and pasture grasses. This species is comprised of two morphologically similar however genetically distinct host strains often known as the corn and rice strains, which may complicate pest administration approaches.
Two molecular markers are generally used to distinguish between strains, nevertheless, discordance between these markers can result in inconclusive pressure identification. Right here, we used double digest restriction website related DNA sequencing to determine diagnostic single nucleotide polymorphisms (SNPs) with alleles distinctive to every pressure.
We then used these strain-specific SNPs to develop 4 real-time PCR primarily based TaqMan assays to quickly and reliably differentiate between strains and interstrain hybrids. These assays present a brand new software for differentiating between strains in field-collected samples, facilitating future research on pressure inhabitants dynamics and interstrain hybridization charges. Understanding the essential ecology of S. frugiperda strains is important to tell future administration methods.
SARS-CoV-2 whole-genome sequencing using reverse complement PCR: For easy, fast and accurate outbreak and variant analysis

Low-copy transgene detection utilizing nested digital PCR for gene doping management

Gene doping is prohibited for honest competitors in human and horse sports activities. One model of gene doping is the administration of an exogeneous gene, known as a transgene, to postnatal people and horses. Though many transgene detection strategies primarily based on quantitative PCR, together with real-time PCR and digital PCR, have been lately developed, it stays troublesome to reliably detect low-copy transgenes.
On this research, we developed and validated a nested digital PCR methodology to particularly detect low-copy transgenes. The nested digital PCR consists of 1) pre-amplification utilizing standard PCR and a couple of) droplet digital PCR detection utilizing a hydrolysis probe.
Utilizing 5, 10, 20, 60, and 120 transgene copies as template, 496.0, 1089.7, 1820.7, 4313.3, and 7840.Zero copies/μL respectively had been detected utilizing our nested digital PCR. Though excessive concentrations of phenol, proteinase Ok, ethanol, EDTA, heparin, and genomic DNA all inhibited pre-amplification, their results on the digital PCR detection had been restricted.
As soon as pre-amplification was profitable, even substitution of bases inside the primers and probes had minimal results on transgene detection. The nested digital PCR developed on this research efficiently detected low-copy transgenes and can be utilized to carry out a qualitative take a look at, indicating its usefulness within the prevention of false positives and false negatives in gene doping detection.

Novel Quadruplex PCR for detecting and genotyping cell colistin resistance genes in human samples

Since 2016, a number of cell colistin resistance (mcr) genes have been recognized worldwide. It is price noting that solely mcr-1, mcr-3, mcr-8, and mcr-10 have been reported remoted immediately from scientific samples which created larger threat to human well being than different mcr gene sorts.
A novel Quadruplex polymerase chain response (Quad-PCR) protocol was developed to detect and genotype transferable colistin-resistance genes (mcr-1, mcr-3, mcr-8, mcr-10) in Enterobacteria for scientific laboratory functions. The protocol was validated by testing 11 scientific isolates of Escherichia coli and three scientific isolates of Klebsiella of human origin, every effectively characterised and prospectively validated.
The Quad-PCR assay confirmed full concordance with whole-genome sequence knowledge and displayed increased sensitivity and 100% specificity. The Quad-PCR assay achieved genotyping of mcr alleles (as singleton and combination with double or triple gene sorts) described in a single take a look at.

Optimum allocation of PCR checks to minimise illness transmission via contact tracing and quarantine

PCR testing is a vital functionality for managing illness outbreaks, however it’s also a restricted useful resource and have to be used rigorously to make sure the data achieve from testing is efficacious. Testing has two broad makes use of for informing public well being coverage, particularly to trace epidemic dynamics and to cut back transmission by figuring out and managing circumstances.
On this work we develop a modelling framework to look at the consequences of take a look at allocation in an epidemic, with a concentrate on utilizing testing to minimise transmission. Utilizing the COVID-19 pandemic for example, we study how the variety of checks performed per day pertains to discount in illness transmission, within the context of logistical constraints on the testing system.
We present that if every day testing is above the routine capability of a testing system, which may trigger delays, then these delays can undermine efforts to cut back transmission via contact tracing and quarantine. This work highlights that the 2 targets of aiming to cut back transmission and aiming to determine all circumstances are totally different, and it’s doable that specializing in one could undermine attaining the opposite.

2 × Rapid Taq Master Mix

P222-02 15 ml (15×1ml)
EUR 144.5

2 × Rapid Taq Master Mix

P222-03 50 ml (50 x 1 ml)
EUR 430

2 × Rapid Taq Master Mix

P222-04 50 ml (10×5ml)
EUR 430

2x Taq PCR Master Mix

A4145-1ML40Rxns 1ML (40Rxns)
EUR 77
Description: Biotechnology

2 × Taq Plus Master Mix

P211-01 5 ml
EUR 58.81

2 × Taq Plus Master Mix

P211-02 15 ml
EUR 162.24

2 × Taq Plus Master Mix

P211-03 50 ml
EUR 507

2X ViRed Taq Master Mix, 100app

CLMM01 each Ask for price

2 × Taq Master Mix (Dye Plus)

P112-01 5 ml
EUR 36.91

2 × Taq Master Mix (Dye Plus)

P112-02 15 ml
EUR 98.42

2 × Taq Master Mix (Dye Plus)

P112-03 50 ml
EUR 290

2 × EpiArt HS Taq Master Mix

EM201-01 1ml
EUR 16

2 × EpiArt HS Taq Master Mix

EM201-02 5 ml (5×1ml)
EUR 70

4 × EpiArt HS Taq Master Mix

EM201-03 15 ml (15×1ml)
EUR 192

Apex 2X Taq Master Mix, Clear

42-133 100 Reactions/Unit
EUR 54.78
Description: 1.5mM MgCl2 (Final Conc.)

Apex 2X Taq Master Mix, Clear

42-134 500 Reactions/Unit
EUR 220.95
Description: 1.5mM MgCl2 (Final Conc.)

Apex 2X Taq Master Mix, Clear

42-134B 1000 Reactions/Unit
EUR 433.29
Description: 1.5mM MgCl2 (Final Conc.)

Apex Taq RED Master Mix, 2X

42-137 100 Reactions/Unit
EUR 58.5
Description: 1.5mM MgCl2 (Final Conc.)

Apex Taq RED Master Mix, 2X

42-138 500 Reactions/Unit
EUR 221.56
Description: 1.5mM MgCl2 (Final Conc.)

Apex Taq RED Master Mix, 2X

42-138B 1000 Reactions/Unit
EUR 437.28
Description: 1.5mM MgCl2 (Final Conc.)

ViPrimePLUS Taq qPCR Master Mix, 150rxns

QLMM11 each Ask for price

Taq PCR Master Mix (2X, Red Dye)

BS9297 1ml
EUR 101.76

Taq PCR Master Mix (2X, Red Dye)

BS9298 5ml
EUR 227.04

Taq PCR Master Mix (2X, Blue Dye)

BS9295 1ml
EUR 101.76

Taq PCR Master Mix (2X, Blue Dye)

BS9296 5ml
EUR 227.04

2X Taq PCR Master Mix (with dye)

K1034-1 1 ml
EUR 16

2X Taq PCR Master Mix (with dye)

K1034-100 100×1 ml
EUR 512

2X Taq PCR Master Mix (with dye)

K1034-20 20×1 ml
EUR 160

2X Taq PCR Master Mix (with dye)

K1034-5 5×1 ml
EUR 48

2X Taq PCR Master Mix (with dye)

K1034-50 50×1 ml
EUR 320

2 × Taq Plus Master Mix (Dye Plus)

P212-01 5 ml
EUR 159.6

2 × Taq Plus Master Mix (Dye Plus)

P212-02 15 ml
EUR 231.6

2 × Taq Plus Master Mix (Dye Plus)

P212-03 50 ml
EUR 466.8

3G Taq Master Mix for PAGE (Red Dye)

P115-01 5 ml
EUR 38.67

3G Taq Master Mix for PAGE (Red Dye)

P115-02 10 × 5 ml
EUR 290

2 × EpiArt HS Taq Master Mix (Dye Plus)

EM202-01 1ml
EUR 16

2 × EpiArt HS Taq Master Mix (Dye Plus)

EM202-02 5 ml (5×1ml)
EUR 70

2 × EpiArt HS Taq Master Mix (Dye Plus)

EM202-03 15 ml (15×1ml)
EUR 192

Taq Pro Universal SYBR qPCR Master Mix

Q712-02 500 rxns (20 μl/rxn)
EUR 161

Taq Pro Universal SYBR qPCR Master Mix

Q712-03 2,500 rxns (20 μl/rxn)
EUR 725

Accuris Taq Master Mix, 2X conc., 1000 reactions

PR1001-1000 1 PC
EUR 375.85

Accuris Taq Master Mix, 2X conc., 200 reactions

PR1001-200 1 PC
EUR 89.71

2 × Taq Plus Master Mix Ⅱ (Dye Plus)

P213-01 5 ml
EUR 58.81

2 × Taq Plus Master Mix Ⅱ (Dye Plus)

P213-02 15 ml
EUR 162.24

2 × Taq Plus Master Mix Ⅱ (Dye Plus)

P213-03 50 ml
EUR 507

Apex Hot Start Taq BLUE Master Mix, 2X

42-143 100 Reactions/Unit
EUR 65.77
Description: 1.5mM MgCl2 (Final)

Apex Hot Start Taq BLUE Master Mix, 2X

42-144 500 Reactions/Unit
EUR 288.71
Description: 1.5mM MgCl2 (Final)

Apex Hot Start Taq BLUE Master Mix, 2X

42-145 1000 Reactions/Unit
EUR 470.2
Description: 1.5mM MgCl2 (Final)

Apex Hot Start Taq BLUE Master Mix, 2X

42-146 2500 Reactions/Unit
EUR 1216.89
Description: 1.5mM MgCl2 (Final), Buffer I

Apex Hot Start Taq BLUE Master Mix, 2X

42-147 100 Reactions/Unit
EUR 61.77
Description: 1.5mM MgCl2 (Final), Buffer II

Apex Hot Start Taq BLUE Master Mix, 2X

42-148 500 Reactions/Unit
EUR 253.97
Description: 1.5mM MgCl2 (Final), Buffer II

Apex Hot Start Taq BLUE Master Mix, 2X

42-149 1000 Reactions/Unit
EUR 473.03
Description: 1.5mM MgCl2 (Final), Buffer II

Apex Hot Start Taq BLUE Master Mix, 2X

42-150 2500 Reactions/Unit
EUR 1072.65
Description: 1.5mM MgCl2 (Final), Buffer II

ViPrimePLUS Taq qPCR Green Master Mix I, 150rxns

QLMM12 each Ask for price

ViPrimePLUS Taq qPCR Green Master Mix II, 150rxns

QLMM17 each Ask for price

2X OneStep Taq ReverseTrans PCR Master Mix, 100apps

RTMM01 each Ask for price

ViPrimePLUS OneStep Taq RT-qPCR Master Mix, 150rxns

QLMM13 each Ask for price

Accuris Taq Master Mix, 2X conc., sample, 5 reactions

PR1001-S 1 PC
EUR 7.31

ViPrimePLUS Taq qPCR Master Mix with ROX, 150rxns

QLMM11-R each Ask for price

Geneaid Hot Start Taq PCR Master Mix with Dye

HSTQ004 each
EUR 2

Geneaid Hot Start Taq PCR Master Mix with Dye

HSTQ100 each
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2X ViRed OneStep Taq ReverseTrans PCR Master Mix, 100apps

RTMM02 each Ask for price

Accuris Taq Master Mix Red Dye, 2X conc., 1000 reactions

PR1001-R-1000 1 PC
EUR 375.85

Accuris Taq Master Mix Red Dye, 2X conc., 200 reactions

PR1001-R-200 1 PC
EUR 90.95

Taqman Master Mix

M1121-500 each
EUR 529.2

Accuris Taq Plus Master Mix, 2X conc., 1000 reactions

PR1001-TP-1000 1 PC
EUR 1182.9

Accuris Taq Plus Master Mix, 2X conc., 200 reactions

PR1001-TP-200 1 PC
EUR 276.6

Accuris Hot Start Taq Master Mix, 2X conc., 1000 reactions

PR1001-HS-1000 1 PC
EUR 678.87

Accuris Hot Start Taq Master Mix, 2X conc., 200 reactions

PR1001-HS-200 1 PC
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ViPrimePLUS Taq qPCR Master Mix with Low ROX, 150rxns

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ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix I, 150rxns

QLMM14 each Ask for price

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix II, 150rxns

QLMM18 each Ask for price

Accuris Taq Master Mix Red Dye, 2X conc., sample, 5 reactions

PR1001-R-S 1 PC
EUR 7.31

Accuris Taq Plus Master Mix, 2X conc., sample, 20 reactions

PR1001-TP-S 1 PC
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ViPrimePLUS Taq qPCR Green Master Mix I with ROX, 150rxns

QLMM12-R each Ask for price

ViPrimePLUS Taq qPCR Green Master Mix II with ROX, 150rxns

QLMM17-R each Ask for price

Taqman Master Mix-ROX

M1124-500 each
EUR 529.2

ViPrimePLUS OneStep Taq RT-qPCR Master Mix with ROX, 150rxns

QLMM13-R each Ask for price

Accuris Hot Start Taq Master Mix Red Dye, 2X conc., 1000 reactions

PR1001-HSR-1000 1 PC
EUR 678.87

Accuris Hot Start Taq Master Mix Red Dye, 2X conc., 200 reactions

PR1001-HSR-200 1 PC
EUR 166.04

Taqman Master Mix-iCycler

M1122-500 each
EUR 529.2

Taqman Master Mix-Multiplex

M1125-500 each
EUR 529.2

ViPrimePLUS Taq qPCR Green Master Mix I with Low ROX, 150rxns

QLMM12-LR each Ask for price

ViPrimePLUS Taq qPCR Green Master Mix II with Low ROX, 150rxns

QLMM17-LR each Ask for price

Ruby Taq Master (2x)

PCR-164L 20 x 1.25ml
EUR 466.7
Description: Ready-to-Use Mixes for PCR

Ruby Taq Master (2x)

PCR-164S 4 x 1,25ml
EUR 116.6
Description: Ready-to-Use Mixes for PCR

Ruby Taq Master (2x)

PCR-164XL 100ml
EUR 1492.9
Description: Ready-to-Use Mixes for PCR

Taqman Master Mix-Low ROX

M1123-500 each
EUR 529.2

Crystal Taq Master (2x)

PCR-166L 20 x 1.25ml
EUR 466.7
Description: Ready-to-Use Mixes for PCR

Crystal Taq Master (2x)

PCR-166S 4 x 1,25ml
EUR 116.6
Description: Ready-to-Use Mixes for PCR

Crystal Taq Master (2x)

PCR-166XL 100ml
EUR 1492.9
Description: Ready-to-Use Mixes for PCR

ViPrimePLUS OneStep Taq RT-qPCR Master Mix with Low Rox, 150rxns

QLMM13-LR each Ask for price

Accuris Hot Start Taq Master Mix Red Dye, 2X conc., sample, 5 reactions

PR1001-HSR-S 1 PC
EUR 7.31

Jade? Master Mix

M1105-500 each
EUR 529.2

qPCR Master Mix

abx460014-1096tests 10 × 96 tests Ask for price

qPCR Master Mix

abx460014-596tests 5 × 96 tests Ask for price

qPCR Master Mix

abx460014-96tests 96 tests
EUR 337.5

Red Load Taq Master (5x)

PCR-108L 5 x 1ml
EUR 207.1

Red Load Taq Master (5x)

PCR-108S 1ml
EUR 51.9

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix I with ROX, 150rxns

QLMM14-R each Ask for price

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix II with ROX, 150rxns

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ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix I with Low ROX, 150rxns

QLMM14-LR each Ask for price

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix II with Low ROX, 150rxns

QLMM18-LR each Ask for price

PCR Master Mix 2X

11140011-1 100 Reaction(s)
EUR 171.48

PCR Master Mix 2X

11140011-2 200 Reaction(s)
EUR 323.39

PCR Master Mix 2X

11140011-3 500 Reaction(s)
EUR 692.46

PCR Master mix (SYBR)

abx460031-1096tests 10 × 96 tests Ask for price

PCR Master mix (SYBR)

abx460031-596tests 5 × 96 tests Ask for price

PCR Master mix (SYBR)

abx460031-96tests 96 tests
EUR 337.5

2 × AceTaq Master Mix

P411-01 1 ml
EUR 142.8

2 × AceTaq Master Mix

P411-02 5 ml
EUR 189.6

2 × AceTaq Master Mix

P411-03 15 ml
EUR 314.4

AceQ Probe Master Mix

Q113-EN02 500 rxns (20 μl/rxn)
EUR 142.33

AceQ Probe Master Mix

Q113-EN03 2,500 rxns (20 μl/rxn)
EUR 634

amfiSure PCR Master Mix

P0311-010 2x50 rxns
EUR 151.2

amfiSure PCR Master Mix

P0311-025 5X50 rxns
EUR 198

amfiSure PCR Master Mix

P0311-050 10X50 rxns
EUR 262.8

amfiSure PCR Master Mix

P0311-100 10x1ml
EUR 343.2

amfiSure PCR Master Mix

P0311-125 15X50 rxns
EUR 333.6

amfiSure PCR Master Mix

P0311-200 20x1ml
EUR 562.8

amfiSure PCR Master Mix

P0311-250 50x50 rxns
EUR 902.4

amfiSure PCR Master Mix

P0311-500 100x50 rxns
EUR 1509.6

2X Pfu Master Mix, 100app

PLMM03 each Ask for price

amfiXpand PCR Master Mix

P0331-010 2X50 rxns
EUR 181.2

amfiXpand PCR Master Mix

P0331-025 5X50 rxns
EUR 327.6

amfiXpand PCR Master Mix

P0331-050 10X50 rxns
EUR 537.6

amfiXpand PCR Master Mix

P0331-250 50x50 rxns
EUR 2247.6

TM 2x Taqman based PCR Master Mix Kit

TBS4002R-2.5 2.5 ml
EUR 129

2X AtTaq Master Mix, 100app

PLMM02 each Ask for price

RT Master Mix for qPCR

HY-K0510 1 mL (100 rxns)
EUR 349.2

AceQ U+ Probe Master Mix

Q113-02 500 rxn (20 μl/rxn)
EUR 320.4

AceQ U+ Probe Master Mix

Q113-03 2500 rxn (20 μl/rxn)
EUR 1010.4

2 × Phanta Max Master Mix

P515-01 1 ml
EUR 45.73

2 × Phanta Max Master Mix

P515-02 5 ml
EUR 124.18

2 × Phanta Max Master Mix

P515-03 15 ml
EUR 348

Hi-TEMP PCR MASTER MIX

MBT119-100R 1 unit
EUR 550.27
Description: Hi-TEMP PCR MASTER MIX

Hi-TEMP PCR MASTER MIX

MBT119-20R 1 unit
EUR 118.47
Description: Hi-TEMP PCR MASTER MIX

Hi-TEMP PCR MASTER MIX

MBT119-50R 1 unit
EUR 283.59
Description: Hi-TEMP PCR MASTER MIX

Fast Probe Master Mix (5000 rxn)

31005-2 50x1ML
EUR 4301
Description: BSA from bovine serum (cow; Bos taurus); recombinant Taq polymerase from E. coli

Fast Probe Master Mix (5000 rxn)

31005-2-1 PK
EUR 4301

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX*

17282 1 mL
EUR 101
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX*

17282-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX*

17283 5 mL
EUR 315
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX*

17283-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

2 × Vazyme LAmp Master Mix

P311-01 1 ml
EUR 26.4

2 × Vazyme LAmp Master Mix

P311-02 5 ml
EUR 97.5

2 × Vazyme LAmp Master Mix

P311-03 15 ml
EUR 261

Hi-Chrom PCR Master Mix

MBT089-100R 1 unit
EUR 62.03
Description: Hi-Chrom PCR Master Mix

Hi-Chrom PCR Master Mix

MBT089-20R 1 unit
EUR 14.39
Description: Hi-Chrom PCR Master Mix

Hi-Chrom PCR Master Mix

MBT089-50R 1 unit
EUR 34.51
Description: Hi-Chrom PCR Master Mix

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX*

17284 1 mL
EUR 101
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX*

17284-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX*

17285 5 mL
EUR 315
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX*

17285-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays.

Apex qPCR Probe Master Mix

42-116P 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, Without ROX, 5ml

Apex qPCR GREEN Master Mix

42-116PG 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, Without ROX, 5ml

Apex qPCR Probe Master Mix

42-117P 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Without ROX, 50ml

Apex qPCR GREEN Master Mix

42-117PG 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Without ROX, 50ml

Apex qPCR Probe Master Mix

42-118P 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, Low ROX, 5ml

Apex qPCR GREEN Master Mix

42-118PG 400 x 25µl Reactions/Unit
EUR 338.88
Description: 2X, Low ROX, 5ml

Apex qPCR Probe Master Mix

42-119P 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Low ROX, 50ml

Apex qPCR GREEN Master Mix

42-119PG 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Low ROX, 50ml

Apex qPCR GREEN Master Mix

42-121PG 4000 x 25µl Reactions/Unit
EUR 3095.57
Description: 2X, High ROX, 50ml

Apex qPCR Probe Master Mix

42-120P 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, High ROX, 5ml

Apex qPCR GREEN Master Mix

42-120PG 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, High ROX, 5ml

Apex qPCR Probe Master Mix

42-121P 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, High ROX, 50ml
To develop an efficient technique, the targets have to be clear and efficiency metrics should match the targets of the testing technique. If metrics don’t match the goals of the technique, then these metrics could incentivise actions that undermine attaining the goals.

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