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A novel microRNA (miRNA) quantification method has been developed using stem-loop RT followed by TaqMan PCR analysis. Stem-loop RT primers are better than conventional ones in terms of RT efficiency and specificity. TaqMan miRNA assays are specific for mature miRNAs and discriminate among related miRNAs that differ by as little as one nucleotide. Furthermore, they are not affected by genomic DNA contamination. Precise quantification is achieved routinely with as little as 25 pg of total RNA for most miRNAs. In fact, the high sensitivity, specificity and precision of this method allows for direct analysis of a single cell without nucleic acid purification. Like standard TaqMan gene expression assays, TaqMan miRNA assays exhibit a dynamic range of seven orders of magnitude. Quantification of five miRNAs in seven mouse tissues showed variation from less than 10 to more than 30,000 copies per cell. This method enables fast, accurate and sensitive miRNA expression profiling and can identify and monitor potential biomarkers specific to tissues or diseases. Stem-loop RT-PCR can be used for the quantification of other small RNA molecules such as short interfering RNAs (siRNAs). Furthermore, the concept of stem-loop RT primer design could be applied in small RNA cloning and multiplex assays for better specificity and efficiency.

A novel microRNA (miRNA) quantification method has been developed using stem-loop RT followed by TaqMan PCR analysisA novel microRNA (miRNA) quantification method has been developed using stem-loop RT followed by TaqMan PCR analysis

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A novel microRNA (miRNA) quantification technique has been developed utilizing stem-loop RT adopted by TaqMan PCR evaluation. Stem-loop RT primers are higher than standard ones by way of RT effectivity

Development of quantitative real-time PCR and digital droplet-PCR assays for rapid and early detection of the spoilage yeasts Saccharomycopsis fibuligera and Wickerhamomyces anomalus in breadDevelopment of quantitative real-time PCR and digital droplet-PCR assays for rapid and early detection of the spoilage yeasts Saccharomycopsis fibuligera and Wickerhamomyces anomalus in bread

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Within the current research, for the primary time, excessive delicate quantitative polymerase chain response (qPCR) and digital droplet polymerase chain response (ddPCR) assays have been developed to detect and quantify

Rapid Electrochemical-Based PCR-Less Microbial Quantification and Antimicrobial Susceptibility Profiling Directly From Blood and Urine With Unknown Microbial Load or SpeciesRapid Electrochemical-Based PCR-Less Microbial Quantification and Antimicrobial Susceptibility Profiling Directly From Blood and Urine With Unknown Microbial Load or Species

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Novel molecular platforms can be found for figuring out (ID) the causative brokers of microbial infections and producing antimicrobial susceptibility testing (AST) profiles, which might inform the acceptable course of

SARS-CoV-2 whole-genome sequencing using reverse complement PCR: For easy, fast and accurate outbreak and variant analysisSARS-CoV-2 whole-genome sequencing using reverse complement PCR: For easy, fast and accurate outbreak and variant analysis

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In the course of the course of the SARS-CoV-2 pandemic studies of mutations with results on spreading and vaccine effectiveness emerged. Giant scale mutation evaluation utilizing fast SARS-CoV-2 Entire Genome

Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

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The 2 mostly used strategies to investigate information from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the enter copy quantity, often by relating the

Category: Psmb6

A novel microRNA (miRNA) quantification method has been developed using stem-loop RT followed by TaqMan PCR analysisA novel microRNA (miRNA) quantification method has been developed using stem-loop RT followed by TaqMan PCR analysis

SARS-CoV-2 whole-genome sequencing using reverse complement PCR: For easy, fast and accurate outbreak and variant analysisSARS-CoV-2 whole-genome sequencing using reverse complement PCR: For easy, fast and accurate outbreak and variant analysis

Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.