The 2 mostly used strategies to investigate information from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the enter copy quantity, often by relating the PCR sign to a normal curve.
Relative quantification relates the PCR sign of the goal transcript in a remedy group to that of one other pattern akin to an untreated management. The two(-Delta Delta C(T)) methodology is a handy option to analyze the relative adjustments in gene expression from real-time quantitative PCR experiments.
The aim of this report is to current the derivation, assumptions, and functions of the two(-Delta Delta C(T)) methodology. As well as, we current the derivation and functions of two variations of the two(-Delta Delta C(T)) methodology which may be helpful within the evaluation of real-time, quantitative PCR information.
A brand new mathematical mannequin for relative quantification in real-time RT-PCR.
Use of the real-time polymerase chain response (PCR) to amplify cDNA merchandise reverse transcribed from mRNA is on the best way to changing into a routine software in molecular biology to review low abundance gene expression. Actual-time PCR is simple to carry out, gives the required accuracy and produces dependable in addition to fast quantification outcomes.
However correct quantification of nucleic acids requires a reproducible methodology and an sufficient mathematical mannequin for information evaluation. This examine enters into the actual matters of the relative quantification in real-time RT-PCR of a goal gene transcript compared to a reference gene transcript. Subsequently, a brand new mathematical mannequin is introduced.
The relative expression ratio is calculated solely from the real-time PCR efficiencies and the crossing level deviation of an unknown pattern versus a management. This mannequin wants no calibration curve. Management ranges had been included within the mannequin to standardise every response run with respect to RNA integrity, pattern loading and inter-PCR variations. Excessive accuracy and reproducibility (<2.5% variation) had been reached in LightCycler PCR utilizing the established mathematical mannequin.
Analyzing real-time PCR information by the comparative C(T) methodology.
Two completely different strategies of presenting quantitative gene expression exist: absolute and relative quantification. Absolute quantification calculates the copy variety of the gene often by relating the PCR sign to a normal curve.
Relative gene expression presents the information of the gene of curiosity relative to some calibrator or inside management gene. A extensively used methodology to current relative gene expression is the comparative C(T) methodology additionally known as the two (-DeltaDeltaC(T)) methodology.
This protocol gives an summary of the comparative C(T) methodology for quantitative gene expression research. Additionally introduced listed below are varied examples to current quantitative gene expression information utilizing this methodology.
Extra modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae.
An necessary current advance within the useful evaluation of Saccharomyces cerevisiae genes is the event of the one-step PCR-mediated approach for deletion and modification of chromosomal genes. This methodology permits very fast gene manipulations with out requiring plasmid clones of the gene of curiosity. We describe right here a brand new set of plasmids that function templates for the PCR synthesis of fragments that permit a wide range of gene modifications.
Utilizing as selectable marker the S. cerevisiae TRP1 gene or modules containing the heterologous Schizosaccharomyces pombe his5+ or Escherichia coli kan(r) gene, these plasmids permit gene deletion, gene overexpression (utilizing the regulatable GAL1 promoter), C- or N-terminal protein tagging [with GFP(S65T), GST, or the 3HA or 13Myc epitope], and partial N- or C-terminal deletions (with or with out concomitant protein tagging).
Due to the modular nature of the plasmids, they permit environment friendly and economical use of a small variety of PCR primers for all kinds of gene manipulations. Thus, these plasmids ought to additional facilitate the fast evaluation of gene operate in S. cerevisiae.
Relative expression software program software (REST) for group-wise comparability and statistical evaluation of relative expression ends in real-time PCR.
Actual-time reverse transcription adopted by polymerase chain response (RT-PCR) is essentially the most appropriate methodology for the detection and quantification of mRNA. It gives excessive sensitivity, good reproducibility and a large quantification vary.
At present, relative expression is more and more used, the place the expression of a goal gene is standardised by a non-regulated reference gene. A number of mathematical algorithms have been developed to compute an expression ratio, primarily based on real-time PCR effectivity and the crossing level deviation of an unknown pattern versus a management.
However all revealed equations and accessible fashions for the calculation of relative expression ratio permit just for the dedication of a single transcription distinction between one management and one pattern. Subsequently a brand new software program software was established, named REST (relative expression software program software), which compares two teams, with as much as 16 information factors in a pattern and 16 in a management group, for reference and as much as 4 goal genes.
The mathematical mannequin used is predicated on the PCR efficiencies and the imply crossing level deviation between the pattern and management group. Subsequently, the expression ratio outcomes of the 4 investigated transcripts are examined for significance by a randomisation check. Herein, growth and utility of REST is defined and the usefulness of relative expression in real-time PCR utilizing REST is mentioned.
Methylation-specific PCR: a novel PCR assay for methylation standing of CpG islands.
Exact mapping of DNA methylation patterns in CpG islands has turn out to be important for understanding numerous organic processes such because the regulation of imprinted genes, X chromosome inactivation, and tumor suppressor gene silencing in human most cancers. We describe a brand new methodology, MSP (methylation-specific PCR), which might quickly assess the methylation standing of nearly any group of CpG websites inside a CpG island, impartial of using methylation-sensitive restriction enzymes.
This assay entails preliminary modification of DNA by sodium bisulfite, changing all unmethylated, however not methylated, cytosines to uracil, and subsequent amplification with primers particular for methylated versus unmethylated DNA. MSP requires solely small portions of DNA, is delicate to 0.1% methylated alleles of a given CpG island locus, and could be carried out on DNA extracted from paraffin-embedded samples.
2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21702 | Bimake | 5 ml | EUR 268.8 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (Low ROX) |
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| B21703 | Bimake | 25 ml | EUR 1027.2 |
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (High ROX) |
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| B21402 | Bimake | 5 ml | EUR 268.8 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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2x SYBR Green qPCR Master Mix (High ROX) |
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| B21403 | Bimake | 25 ml | EUR 1027.2 |
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Description: 2x SYBR Green qPCR master mix (High ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR |
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AceQ qPCR SYBR® Green Master Mix |
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| Q111-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 265.2 |
AceQ qPCR SYBR® Green Master Mix |
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| Q111-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 775.2 |
HotStart™ 2X SYBR Green qPCR Master Mix |
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| K1070-25 | ApexBio | 25ml | EUR 420 |
HotStart™ 2X SYBR Green qPCR Master Mix |
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| K1070-5 | ApexBio | 5ml | EUR 120 |
HotStart™ 2X SYBR Green qPCR Master Mix |
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| K1070-50 | ApexBio | 50ml | EUR 840 |
HotStart™ Universal 2X SYBR Green qPCR Master Mix |
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| K1170-25 | ApexBio | 25ml | EUR 420 |
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Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox) |
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HotStart™ Universal 2X SYBR Green qPCR Master Mix |
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| K1170-5 | ApexBio | 5ml | EUR 120 |
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Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox) |
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HotStart™ Universal 2X SYBR Green qPCR Master Mix |
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| K1170-50 | ApexBio | 50ml | EUR 840 |
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Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox) |
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ChamQ SYBR qPCR Master Mix |
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| Q311-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 96.44 |
ChamQ SYBR qPCR Master Mix |
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| Q311-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 434.8 |
ChamQ SYBR Color qPCR Master Mix |
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| Q411-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 112.5 |
ChamQ SYBR Color qPCR Master Mix |
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| Q411-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 507.2 |
AceQ Universal SYBR qPCR Master Mix |
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| Q511-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 265.2 |
AceQ Universal SYBR qPCR Master Mix |
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| Q511-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 775.2 |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 265.2 |
ChamQ Universal SYBR qPCR Master Mix |
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| Q711-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 775.2 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
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| Q321-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 96.44 |
ChamQ SYBR qPCR Master Mix (Without ROX) |
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| Q321-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 434.8 |
Taq Pro Universal SYBR qPCR Master Mix |
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| Q712-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 128.8 |
Taq Pro Universal SYBR qPCR Master Mix |
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| Q712-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 580 |
miRNA Universal SYBR® qPCR Master Mix |
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| MQ101-01 | Vazyme | 125 rxn(20 μl/rxn) | EUR 165.6 |
miRNA Universal SYBR® qPCR Master Mix |
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| MQ101-02 | Vazyme | 500 rxn(20 μl/rxn) | EUR 290.4 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
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| Q331-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 96.44 |
ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
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| Q331-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 434.8 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
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| Q341-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 96.44 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
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| Q341-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 434.8 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
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| Q421-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 112.5 |
ChamQ SYBR Color qPCR Master Mix (Without ROX) |
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| Q421-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 507.2 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
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| Q431-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 112.5 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
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| Q431-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 507.2 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
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| Q441-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 112.5 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
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| Q441-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 507.2 |
TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17270-1mL | AAT Bioquest | 1 mL | EUR 99 |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17271-5mL | AAT Bioquest | 5 mL | EUR 308 |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17272-1mL | AAT Bioquest | 1 mL | EUR 99 |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17273-5mL | AAT Bioquest | 5 mL | EUR 308 |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17274-1mL | AAT Bioquest | 1 mL | EUR 99 |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17275-5mL | AAT Bioquest | 5 mL | EUR 308 |
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Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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Genious 2X SYBR Green Fast qPCR Mix |
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| RK21204 | Abclonal | 1 mL | Ask for price |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17276-1mL | AAT Bioquest | 1 mL | EUR 99 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX* |
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| 17277-5mL | AAT Bioquest | 5 mL | EUR 308 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17278-1mL | AAT Bioquest | 1 mL | EUR 99 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX* |
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| 17279-5mL | AAT Bioquest | 5 mL | EUR 308 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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2X Universal SYBR Green Fast qPCR Mix |
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| RK21203 | Abclonal | 1 mL | Ask for price |
TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17280-1mL | AAT Bioquest | 1 mL | EUR 99 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX* |
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| 17281-5mL | AAT Bioquest | 5 mL | EUR 308 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR. |
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TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
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| 17270 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX* |
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| 17271 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
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| 17272 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX* |
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| 17273 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
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| 17274 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX* |
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| 17275 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
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| 17276 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX* |
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| 17277 | AAT Bioquest | 5 mL | EUR 308 |
lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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| M-PCR-173L | MiTeGen | 960 reactions x 20 µl | EUR 897 |
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Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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| M-PCR-173S | MiTeGen | 192 reactions x 20 µl | EUR 224 |
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Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain |
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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
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| 17278 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX* |
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| 17279 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
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| 17280 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX* |
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| 17281 | AAT Bioquest | 5 mL | EUR 308 |
BrightCycle Universal SYBR Green qPCR Mix with UDG |
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| RK21219 | Abclonal | 5mL | EUR 475 |
Genious 2X SYBR GREEN FAST qPCR MIX (No ROX) |
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| RK21205 | Abclonal | 1 ml | Ask for price |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-001 | GenDepot | 1ml | EUR 336 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-002 | GenDepot | 2x1ml | EUR 578.4 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-005 | GenDepot | 5x1ml | EUR 993.6 |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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| P0346-010 | GenDepot | 10x1ml | EUR 1632 |
RT Master Mix for qPCR |
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| HY-K0510 | MedChemExpress | 1 mL (100 rxns) | EUR 349.2 |
AceQ qPCR Probe Master Mix |
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| Q112-02 | Vazyme | 500 rxn (20 μl/rxn) | EUR 265.2 |
AceQ qPCR Probe Master Mix |
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| Q112-03 | Vazyme | 2500 rxn (20 μl/rxn) | EUR 775.2 |
1-Step 2X RT-PCR Master Mix-SYBR Green-HY (High Yield) |
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| W141 | 101Bio | 200 rxn | EUR 199 |
HotStart PCR Master mix (2X, Green Dye) |
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| BS9291 | Bio Basic | 5X1mL, 5ml | EUR 251.4 |
HotStart PCR Master mix (2X, Green Dye) |
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| BS9292 | Bio Basic | 1ml | EUR 115.68 |
1-Step 2X RT-PCR Master Mix-SYBR Green-HP (High Processibility) |
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| W142 | 101Bio | 200 rxn | EUR 199 |
Entrans 2X qPCR Probe Master Mix |
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| RK21208 | Abclonal | 40 RXN | Ask for price |
Fast Plus EvaGreen qPCR Master Mix (5000 rxn) |
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| 31020-2 | Biotium | 50x1mL | EUR 4291.2 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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Fast Plus EvaGreen qPCR Master Mix (500 rxn) |
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| 31020-1 | Biotium | 5x1mL | EUR 524.4 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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EZGreen 2x qPCR Master Mix, 40Rxns-50ul |
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| A4157-1ML40Rxns | Biomatik Corporation | 1ML (40Rxns) | EUR 55 |
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Description: Biotechnology |
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Green-2-Go qPCR Mastermix (500X20ul Rxn) |
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| QPCR004-S | Bio Basic | 4X1.25ml | EUR 303.25 |
ABScript III RT Master Mix for qPCR |
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| RK20428 | Abclonal | 10RXN | EUR 82.5 |
ABScript Neo RT Master Mix for qPCR |
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| RK20432 | Abclonal | 100RXN | EUR 17.5 |
Entrans 2X qPCR Probe Master Mix V2 |
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| RK21212 | Abclonal | 50RXN | EUR 67.5 |
Fast EvaGreen Master Mix for qPCR (5000 rxn) |
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| 31003-2 | Biotium | 50x1ML | EUR 3739.2 |
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Description: Minimum order quantity: 1 unit of 50x1ML |
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Fast EvaGreen Master Mix for qPCR(200 rxn) |
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| 31003 | Biotium | 2x1ML | EUR 244.8 |
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Description: Minimum order quantity: 1 unit of 2x1ML |
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Fast EvaGreen Master Mix for qPCR (500 rxn) |
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| 31003-1 | Biotium | 5x1ML | EUR 463.2 |
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Description: Minimum order quantity: 1 unit of 5x1ML |
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Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn) |
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| QPCR001-R | Bio Basic | 4X1.25ml | EUR 303.25 |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (2000 reactions) |
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| 31041-20mL | Biotium | 2x10mL | EUR 592.8 |
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Description: Minimum order quantity: 1 unit of 2x10mL |
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Green-2-Go qPCR Mastermix-iCycler (500X20ul Rxn) |
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| QPCR003-iC | Bio Basic | 4X1.25ml | EUR 303.25 |
RT Master Mix for qPCR (gDNA digester plus) |
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| HY-K0511 | MedChemExpress | 1 mL (100 rxns) | EUR 378 |
Entrans 2X qPCR Probe Master Mix with UDG |
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| RK21222 | Abclonal | 50RXN | EUR 71 |
Green-2-Go qPCR Mastermix-no Dye (500X20ul Rxn) |
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| QPCR006-NODYE | Bio Basic | 4X1.25ml | EUR 326.22 |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (500 reactions) |
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| 31041-1 | Biotium | 5x1mL | EUR 326.4 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (100 reactions) |
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| 31041-T | Biotium | 1mL | EUR 136.8 |
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Description: Minimum order quantity: 1 unit of 1mL |
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Genorise® 2 x qPCR HotStart Master Mix |
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| GR108002 | Genorise Scientific | 2.0 ml | EUR 279 |
Green-2-Go qPCR Mastermix-Low ROX (500X20ul Rxn) |
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| QPCR002-L | Bio Basic | 4X1.25ml | EUR 303.25 |
TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17282-1mL | AAT Bioquest | 1 mL | EUR 99 |
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Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
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TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17283-5mL | AAT Bioquest | 5 mL | EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
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TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX* |
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| 17284-1mL | AAT Bioquest | 1 mL | EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
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TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *Low ROX* |
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| 17285-5mL | AAT Bioquest | 5 mL | EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
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TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17286-1mL | AAT Bioquest | 1 mL | EUR 99 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
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TAQuestâ„¢ qPCR Master Mix for TaqMan Probes *High ROX* |
|||
| 17287-5mL | AAT Bioquest | 5 mL | EUR 308 |
|
Description: TAQuestâ„¢ qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR compatible with TaqMan gene expression assays. |
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TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17282 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17283 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
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| 17284 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX* |
|||
| 17285 | AAT Bioquest | 5 mL | EUR 308 |
Fast Plus EvaGreen qPCR Master Mix (trial size, 100 rxn) |
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| 31020-T | Biotium | 1mL | EUR 160.8 |
|
Description: Minimum order quantity: 1 unit of 1mL |
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TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17286 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX* |
|||
| 17287 | AAT Bioquest | 5 mL | EUR 308 |
Green-2-Go TaqProbe qPCR Mastermix-no Dye (500X20ul Rxn) |
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| QPCR005-NODYE | Bio Basic | 4X1.25ml | EUR 303.25 |
iGreen 2X qPCR Master Mix with Low-ROX, 500Rxns-20ul |
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| A4183-4x125ML500Rxns | Biomatik Corporation | 4x1.25ML (500Rxns) | EUR 200.2 |
|
Description: Biotechnology |
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TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
|||
| 17288-1mL | AAT Bioquest | 1 mL | EUR 99 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
|||
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
|||
| 17289-5mL | AAT Bioquest | 5 mL | EUR 308 |
|
Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
|||
TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
|||
| 17290-1mL | AAT Bioquest | 1 mL | EUR 99 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
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TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
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| 17291-5mL | AAT Bioquest | 5 mL | EUR 308 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
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TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17292-1mL | AAT Bioquest | 1 mL | EUR 99 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
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TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17293-5mL | AAT Bioquest | 5 mL | EUR 308 |
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Description: TAQuestâ„¢ FAST qPCR Master Mix for TaqMan Probes is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR well suited to use for TaqMan gene expression assays. |
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Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn) |
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| 31014-1 | Biotium | 5x1mL | EUR 524.4 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn) |
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| 31014-2 | Biotium | 50x1mL | EUR 4291.2 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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Fast Plus EvaGreen qPCR Master Mix with high Rox (500 rxn) |
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| 31015-1 | Biotium | 5x1mL | EUR 524.4 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast Plus EvaGreen qPCR Master Mix with high Rox (5000 rxn) |
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| 31015-2 | Biotium | 50x1mL | EUR 4291.2 |
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Description: Minimum order quantity: 1 unit of 50x1mL |
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TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17288 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX* |
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| 17289 | AAT Bioquest | 5 mL | EUR 308 |
qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials |
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| S190 | GeneOn | 100 rcs (2,5 ml) | EUR 165.6 |
qPCR Master Mix DLP1 (SafeLine: dUTP), packed in 1,25 ml vials |
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| S190L | GeneOn | 500 rcs (12,5 ml) | EUR 627.6 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
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| 17290 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX* |
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| 17291 | AAT Bioquest | 5 mL | EUR 308 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17292 | AAT Bioquest | 1 mL | EUR 99 |
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX* |
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| 17293 | AAT Bioquest | 5 mL | EUR 308 |
Fast EvaGreen Master Mix for qPCR, trial size (100 rxn) |
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| 31003-T | Biotium | 1ML | EUR 147.6 |
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Description: Minimum order quantity: 1 unit of 1ML |
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ABScript III RT Master Mix for qPCR with gDNA Remover |
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| RK20429 | Abclonal | 10RXN | EUR 20 |
ABScript Neo RT Master Mix for qPCR with gDNA Remover |
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| RK20433 | Abclonal | 100RXN | EUR 30 |
Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL) |
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| 31014-T | Biotium | 1mL | EUR 160.8 |
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Description: Minimum order quantity: 1 unit of 1mL |
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Fast Plus EvaGreen qPCR Master Mix with High Rox (100 rxn): (1mL) |
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| 31015-T | Biotium | 1mL | EUR 160.8 |
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Description: Minimum order quantity: 1 unit of 1mL |
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qPCR Master Mix DLP2 (SafeLine: dUTP, UNG), packed in 1,25 ml vials |
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| S200 | GeneOn | 100 rcs (2,5 ml) | EUR 190.8 |
qPCR Master Mix DLP2 (SafeLine: dUTP, UNG), packed in 1,25 ml vials |
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| S200L | GeneOn | 500 rcs (12,5 ml) | EUR 734.4 |
Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions) |
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| 9-31045 | Biotium |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (500 reactions) |
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| 31042-1 | Biotium | 5x1mL | EUR 326.4 |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (200 reactions) |
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| 31042-20mL | Biotium | 2x10mL | EUR 592.8 |
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Description: Minimum order quantity: 1 unit of 2x10mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix with ROX (100 reactions) |
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| 31042-T | Biotium | 1mL | EUR 136.8 |
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Description: Minimum order quantity: 1 unit of 1mL |
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (High ROX) (500 reactions) |
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| 9-31046 | Biotium |
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Description: Minimum order quantity: 1 unit of 5x1mL |
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Fast Plus EvaGreen Master Mix for qPCR with Rox (200 rxn) |
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| 31014 | Biotium | 2x1mL | EUR 273.6 |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials |
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| S150 | GeneOn | 100 rcs (2,5 ml) | EUR 165.6 |
qPCR Master Mix E1 (SafeLine: EvaGreen, dUTP), packed in 1,25 ml vials |
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| S150L | GeneOn | 500 rcs (12,5 ml) | EUR 627.6 |
qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-372L | MiTeGen | 10 x 1,25 ml | EUR 818 |
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Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-372S | MiTeGen | 2 x 1,25 ml | EUR 205 |
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Description: qPCR SybrMasterMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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CRISPY™ Master Mix for qPCR-based Gene Editing Quantitation |
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| CRISPY100A-1 | SBI | 200 rxn | EUR 412 |
qPCR Master Mix E2 (SafeLine: EvaGreen, dUTP, UNG), packed in 1,25 ml vials |
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| S160 | GeneOn | 100 rcs (2,5 ml) | EUR 190.8 |
qPCR Master Mix E2 (SafeLine: EvaGreen, dUTP, UNG), packed in 1,25 ml vials |
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| S160L | GeneOn | 500 rcs (12,5 ml) | EUR 734.4 |
Fast Plus EvaGreen Master Mix for qPCR with High Rox (200 rxn) |
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| 31015 | Biotium | 2x1mL | EUR 273.6 |
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Description: Minimum order quantity: 1 unit of 2x1mL |
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qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-373L | MiTeGen | 10 x 1,25 ml | EUR 818 |
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Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-373S | MiTeGen | 2 x 1,25 ml | EUR 205 |
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Description: qPCR SybrMaster lowROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-375L | MiTeGen | 10 x 1,25 ml | EUR 982 |
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Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-375S | MiTeGen | 2 x 1,25 ml | EUR 246 |
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Description: qPCR SybrMaster UNGMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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2×SYBR-Green-qPCR-Mix |
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| E37F021 | EnoGene | 10×5mL | EUR 1360 |
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Description: DsDNA,-ssDNA,-RNA-quantitative-detection-kit |
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qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials |
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| S210 | GeneOn | 100 rcs (2,5 ml) | EUR 165.6 |
qPCR Master Mix DLP3 (SafeLine: dUTP, 500 nM ROX), packed in 1,25 ml vials |
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| S210L | GeneOn | 500 rcs (12,5 ml) | EUR 627.6 |
SYBR Green I |
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| B1747-25 | Biovision | each | EUR 1396.8 |
SYBR Green I |
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| B1747-5 | Biovision | each | EUR 464.4 |
qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-374L | MiTeGen | 10 x 1,25 ml | EUR 818 |
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Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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| M-PCR-374S | MiTeGen | 2 x 1,25 ml | EUR 205 |
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Description: qPCR SybrMaster highROXMaster mix for real-time qPCR with SYBR® Green fluorescent DNA stain** shipped ice packs - must be shipped via overnight service |
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qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
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| S175 | GeneOn | 100 rcs (2,5 ml) | EUR 165.6 |
qPCR Master Mix E5 (EvaGreen, dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
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| S175L | GeneOn | 500 rcs (12,5 ml) | EUR 627.6 |
qPCR Master Mix DLP5 (SafeLine: dUTP, 100 nM LOW ROX), packed in 1,25 ml vials |
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| S215 | GeneOn | 100 rcs (2,5 ml) | EUR 190.8 |
MSP eliminates the false constructive outcomes inherent to earlier PCR-based approaches which relied on differential restriction enzyme cleavage to tell apart methylated from unmethylated DNA. On this examine, we exhibit using MSP to determine promoter area hypermethylation adjustments related to transcriptional inactivation in 4 necessary tumor suppressor genes (p16, p15, E-cadherin, and von Hippel-Lindau) in human most cancers.
